XANTHENE DYES-MEDIATED IN VITRO PHOTODYNAMIC
TREATMENT OF CANCER AND NON-CANCER CELL LINES
Autoři:
Lukáš Malina 1,2; Kateřina Bartoň Tománková 1,2; Barbora Hošíková 1; Jana Jiravová 1,2; Jakub Hošík 1; Jana Zapletalová 1; Hana Kolářová 1
Působiště autorů:
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
1; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
2
Vyšlo v časopise:
Lékař a technika - Clinician and Technology No. 3, 2020, 50, 114-121
Kategorie:
Original research
doi:
https://doi.org/10.14311/CTJ.2020.3.05
Souhrn
Rose bengal and erythrosin B are xanthene dyes mainly known and used as antimicrobial agents, but due to their photodynamic activity they are also potential photosensitizers for cancer photodynamic therapy. The aim of this work is to study a photodynamic efficacy of rose bengal and erythrosin B against human skin melanoma and mouse fibroblast cell lines, compare them with each other and find out their photodynamic properties induced by light emitting diodes with total light dose of 5 J/cm2. To fully identify and understand photodynamic properties of both potentially effective photo-sensitizers, a set of complex in vitro tests such as cell cytotoxic assay, measurement of reactive oxygen species production, mitochondrial membrane potential change assay, mode of cell death determination or comet assay were made. Although both photosensitizers proved to have similar properties such as increasing production of reactive oxygen species with the higher concentration, predominance of necrotic mode of death or genotoxicity, the more effective photosensitizer was rose bengal because its EC50 was over 20 times lower for both cell lines than in case of erythrosine B.
Zdroje
- Li X, Lee S, Yoon J. Supramolecular photosensitizers reju-venate photodynamic therapy. Chemical Society Reviews. 2018;47(4):1174–88. DOI: 10.1039/C7CS00594F
- Kim M, Jung HY, Park HJ. Topical PDT in the treatment of benign skin diseases: principles and new applications. Inter-national journal of molecular sciences. 2015;16:23259–78. DOI: 10.3390/ijms161023259
- Abrahamse H, Hamblin MR. New photosensitizers for photo-dynamic therapy. Biochemical Journal. 2016;473(4):347–64. DOI: 10.1042/BJ20150942
- Bayona AM, Mroz P, Thunshelle C, Hamblin MR. Design fea-tures for optimization of tetrapyrrole macrocycles as antimicro-bial and anticancer photosensitizers. Chemical biology and drug design. 2017;89(2):192–206. DOI: 10.1111/cbdd.12792
- Hong EJ, Choi DG, Shim MS. Targeted and effective photo-dynamic therapy for cancer using functionalized nanomaterials. Acta Pharmaceutica Sinica B. 2016;6(4):297–307. DOI: 10.1016/j.apsb.2016.01.007
- Allison RR, Moghissi K. Oncologic photodynamic therapy: Clinical strategies that modulate mechanisms of action. Photo-diagnosis and photodynamic therapy. 2013;10(4):331–41. DOI: 10.1016/j.pdpdt.2013.03.011
- Nagata JY, Hioka N, Kimura E, Batistela VR, Terada RS, Graciano AX, et al. Antibacterial photodynamic therapy for dental caries: Evaluation of the photosensitizers used and light source properties. Photodiagnosis and photodynamic therapy. 2012;9(2):122–31. DOI: 10.1016/j.pdpdt.2011.11.006
- Qiu H, Tan M, Ohulchanskyy TY, Lovell JF, Chen G. Recent Progress in Upconversion Photodynamic Therapy. Nanomate-rials. 2018;8(5):344. DOI: 10.3390/nano8050344
- Rajendran M. Quinones as photosensitizer for photodynamic therapy: ROS generation, mechanism and detection methods. Photodiagnosis and photodynamic therapy. 2016;13:175–87. DOI: 10.1016/j.pdpdt.2015.07.177
- Wen X, Li Y, Hamblin MR. Photodynamic therapy in derma-tology beyond non-melanoma cancer: An update. Photodiag-nosis and photodynamic therapy. 2017;19:140–52. DOI: 10.1016/j.pdpdt.2017.06.010
- Meng Z, Hou W, Zhou H, Zhou L, Chen H, Wu C. Therapeutic Considerations and Conjugated Polymer-Based Photosensi-tizers for Photodynamic Therapy. Macromolecular Rapid Com-munications. 2017;39(5). DOI: 10.1002/marc.201700614
- Kwiatkowski S, Knap B, Przystupski D, Saczko J, Kedzierska E, Knap-Czop K, et al. Photodynamic therapy – mechanisms, photosensitizers and combinations. Biomedicine and Pharma-cotherapy. 2018;106:1098–1107. DOI: 10.1016/j.biopha.2018.07.049
- Mokwena MG, Kruger CA, Ivan MT, Heidi A. A review of nanoparticle photosensitizer drug delivery uptake systems for photodynamic treatment of lung cancer. Photodiagnosis and photodynamic therapy. 2018;22:147–54. DOI: 10.1016/j.pdpdt.2018.03.006
- Dabrzalska M, Janaszewska A, Zablocka M, Mignani S, Majoral JP, Klajnert-Maculewicz. Cationic Phosphorus Dendrimer Enhances Photodynamic Activity of Rose Bengal against Basal Cell Carcinoma Cell Lines. Molecular Pharma-ceutics. 2017;14:1821–30. DOI: 10.1021/acs.molpharmaceut.7b00108
- Buck ST, Bettanin F, Orestes E, Homem-de-Mello P, Imasato H, Viana RB, et al. Photodynamic Efficiency of Xanthene Dyes and Their Phototoxicity against a Carcinoma Cell Line: A Computational and Experimental Study. Journal of Chemis-try. 2017. DOI: 10.1155/2017/7365263
- McEwan C, Nesbitt H, Nicholas D, Kavanagh OS, McKenna K, Loan P, et al. Comparing the efficacy of photodynamic and sonodynamic therapy in non-melanoma and melanoma skin cancer. Bioorganic and Medicinal Chemistry. 2016;24(13): 3023–8. DOI: 10.1016/j.bmc.2016.05.015
- Rossoni RD, Junqueira JC, Santos EL, Costa AC, Jorge AO. Comparison of the efficacy of Rose Bengal and erythrosin in photodynamic therapy against Enterobacteriaceae. Lasers in Medical Science. 2010;25:581–6.
DOI: 10.1007/s10103-010-0765-1 - Calori IR, Pellosi DS, Vanzin D, Cesar GB, Pereira PC, Politi MJ, et al. Distribution of Xanthene Dyes in DPPC Vesicles: Rationally Accounting for Drug Partitioning Using a Mem-brane Model. Journal of the Brazilian Chemical Society. 2016;27(11):1938–48. DOI: 10.5935/0103-5053.20160079
- Kniebühler G, Pongratz T, Betz CS, Göke B, Sroka R, Stepp H, Schirra J. Photodynamic therapy for cholangiocarcinoma using low dose mTHPC (Foscan). Photodiagnosis and photodynamic therapy. 2013;10:220–8. DOI: 10.1016/j.pdpdt.2012.12.005
- Wang Y, Lin Y, Zhang H, Zhu J. A photodynamic therapy com-bined with topical 5-aminolevulinic acid and systemic hemato-porphyrin derivative is more efficient but less phototoxic for cancer. Journal of Cancer Research and Clinical Oncology. 2015;142(4):813–21. DOI: 10.1007/s00432-015-2066-3
- Srivastav AK, Mujtaba SF, Dwivedi A, Amar SK, Goyal S, Verma A, et al. Photosensitized rose Bengal-induced photo-toxicity on human melanoma cell line under natural sunlight exposure. Journal of Photochemistry and Photobiology B: Bio-logy. 2016;156:87–99. DOI: 10.1016/j.jphotobiol.2015.12.001
- Pellosi DS, Estevao BM, Semensato J, Severino D, Baptista MS, Politi MJ, et al. Photophysical properties and interactions of xanthene dyes in aqueous micelles. Journal of Photo-chemistry and Photobiology A: Chemistry. 2012;247:8–15. DOI: 10.1016/j.jphotochem.2012.07.009
- Song L, Li C, Zou Y, Dai F, Luo X, Wang B, et al. O2 and Ca2+ Fluxes as Indicators of Apoptosis Induced by Rose Bengal–Mediated Photodynamic Therapy in Human Oral Squamous Carcinoma Cells. Photomedicine and Laser Surgery. 2015; 33(5):258–65. DOI: 10.1089/pho.2014.3863
- Rabe SZ, Mousavi SH, Tabasi N, Rastin M, Rabe SZ, Siadat Z, Mahmoudi M. Rose Bengal suppresses gastric cancer cell pro-liferation via apoptosis and inhibits nitric oxide formation in macrophages. Journal of Immunotoxicology. 2014;11(4):367–75. DOI: 10.3109/1547691X.2013.853715
- Liu Y, Meng X, Bu W. Upconversion-based photodynamic cancer therapy. Coordination Chemistry Reviews. 2019;379: 82–98. DOI: 10.1016/j.ccr.2017.09.006
- Garg AD, Bose M, Ahmed MI, Bonass WA, Wood SR. In
Vitro Studies on Erythrosine-Based Photodynamic Therapy of Malignant and Pre-Malignant Oral Epithelial Cells. PLoS ONE. 2012;7(4):e34475. DOI: 10.1371/journal.pone.0034475 - Calzavara-Pinton PG, Venturini M, Sala R. Photodynamic therapy: update 2006 Part 1: Photochemistry and photobiology. Journal of the European Academy of Dermatology and Vene-reology. 2007;21(3):293–302.
DOI: 10.1111/j.1468-3083.2006.01902.x
Štítky
BiomedicínaČlánek vyšel v časopise
Lékař a technika
2020 Číslo 3
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XANTHENE DYES-MEDIATED IN VITRO PHOTODYNAMIC
TREATMENT OF CANCER AND NON-CANCER CELL LINES