Apheresis red cell concentrates - evaluation of apoptosis markers in concentrates obtained from two devices
Authors:
R. Procházková 1; C. Andrýs 2; J. Krejsek 2; M. Bláha 3
Authors‘ workplace:
Transfuzní oddělení, KN Liberec, 2Ústav klinické imunologie a alergologie LF UK a FN Hradec Králové
1; Oddělení klinické hematologie LF UK a FN Hradec Králové
3
Published in:
Transfuze Hematol. dnes,14, 2008, No. 4, p. 159-165.
Category:
Comprehensive Reports, Original Papers, Case Reports
Overview
Background:
Cells apoptosis is induced by the collection and storage of red blood cell concentrates. Aim of our study was to evaluate red cells storage lesions in red blood cells concentrates collected by two separators with different technology of apheresis.
Methods:
We prepared 40 TU leukoreduced RBC (MCS RBC-WBC reduced) and 40 TU RBC (MCS RBC) using separator Haemonetics MCS⁺. Using device Trima Accel we prepared 19 TU leukoreduced RBC (Trima RBC) by combined RBC + PLT collections. RBC were compared using evaluation of plasma Hb, pH, LDH, annexin V levels immediately after collection and at the end of expiration. The results between products from both devices and whole blood leukoreduced RBC (WB-RBC) were compared.
Results:
The highest increase in the levels of plasma Hb, K⁺, LDH and annexin V was found in non-leukoreduced MCS RBC (plasma Hb: 47 ± 10 to 216 ± 65 mg/TU, v. Trima RBC p < 0.001, v. WB-RBC: p < 0.01; K⁺: 1.2 ± 0.3 to 55.2 ±9.1 mmol/L, p < 0.001; LDH: 2.44 ± 0.5 to 18.8 ± 4.39 μkat/L, p < 0.001; annexin V: 5.7 ± 4.2 to 31.2 ± 3.8 ng/mL, p < 0.001). MCS RBC – WBC reduced in comparison with Trima RBC showed higher rise in the levels of plasma Hb and LDH (p < 0.001). The rise in the levels of K⁺ and annexin V were comparable (p = 0.19/0.33) in these products. Leukoreduced RBC from both separators revealed comparable rise in plasma Hb and K⁺ concentrations compared to WB-RBC. MCS RBC – WBC reduced showed higher increase of annexin V in comparison with WB-RBC (p < 0.01).
Conclusions:
The quality of apheresis red blood concentrates was high, blood cells were not damaged by apheresis. Based on the highest rise of all markers in nonleukoreduced apheresis RBC it could be concluded that development of red blood cells storage lesions is not influenced by apheresis technology. Significant factors are storage conditions and leukocyte content in apheresis products.
Key words:
red blood cells concentrates, red blood cells storage lesions, annexin V
Sources
1. Moog R, Franck V, Pierce JA,¨Muller N. Evaluation of a concurrent multicomponent collection system for the collection and storage of WBC – reduced RBC apheresis concentrates. Transfusion 2001; 41: 1159–1164.
2. Moog R, Bartsch R, Muller N. Concurrent collection of in-line filtered platelets and red blood cells by apheresis. Ann Haematol 2002; 81: 322–325.
3. Guide to the preparation, use and quality assurance of blood components. 10th ed. Strasburg, Council of Europe Publishing, 2004.
4. Elfath MD, Whitley P, Jacobson MS, et al. Evaluation of an automated system for the collection of packed RBCs, platelets, and plasma. Transfusion 2000; 40: 1214–1222.
5. Smith J.W., Gilcher R.O. The future of automated red blood cell collection. Transfus Apher Sci 2006; 34: 219–226.
6. Dzik WH. Apoptosis, TGF beta and transfusion – related immunosupresion: Biologic versus clinical efects. Transf Apher Sci 2003; 29: 127–129.
7. Lang F, Foller M, Lang KS, et al. Ion channels in cell proliferation and apoptotic cell death. J Membrane Bioll 2005; 205: 147–157.
8. Leitner GC, Stohlawetz P J, Stiegler G, et al. Quality of packed red blood cells and platelet concentrates by multicomponent collection using the MCS Plus device. Journal of Clinical Apheresis 2003; 18: 21–25.
9. Solheim BG, Flesland O, Seghatchian J, Brosstad F. Clinical implications of red blood cell and platelet storage lesions: an overview. Transfus Apher Sci 2004; 31: 185–189.
10. Gibson JG, Murphy WP, Scheitlin WA, et al. The influence of intracellular factors involved in the collection of blood in ACD on maintenance of red cell viability during refrigerated storage. Am J Clin Pathol 1956; 26: 858–873.
11. Bessos H, Seghatchian J. Red cell storage lesions: The potential impact of storage induced CD47 decline on immunomodulation and the survival of leucofiltered red cells. Transf Apher Sci 2005; 32: 227–232.
12. Wolfe LC. The membrane and the lesions of storage in preserved red cells. Transfusion 1985; 25: 185–202.
13. Matthes G A. Options and cost effectiveness of multicomponent blood collection. Transfus Apher Sci 2002; 27: 115–121.
14. Müller – Steinhardt M, Janetzko K, Kandler R, Flament J, Kirchner H, Kluter H. Impact of various red cell concentrate preparation methods on the efficiency of prestorage white cell filtration and red cells during storage for 42 days. Transfusion 1997; 37: 1137–1142.
15. Llohn A, Vetlesen A, Fagerhol MK, Kjeldsen-Kragh J. The effect of pre-storage cooling on 2,3-DPG levels in red cells stored in SAG-M. Transfus Apher Sci 2005; 33: 113–118.
16. Holme S, Elfath MD., Whitley P. Evaluation of in vivo and in vitro quality of apheresis-collected RBC stored for 42 days. Vox Sanq 1998; 75: 212–217.
17. Krailadsiri P, Seghatchian J. Effect of processing and storage on platelet activation, cellular injury and microvesiculation. Transfus Apher Sci 2001, 24: 237–238.
18. Seghatchian J, Krailadsiri P. Red cell storage lesion assesed by the levels of potassium, hemoglobin and annexin V in supernatantant. Transfus Apher Sci 2002; 26: 121–127.
19. Hornsey V, Drummond O, Mac Gregor I, et al. Leucofiltration, retention/generation of soluble prion and Annexin V and storage of blood components. Transfus Sci 2000; 22: 75–76.
20. Lin SJ, Tzeng CH, Hao HY, et al. Cytokine release in febrile non-haemolytic red cell transfusion reactions. Vox Sanq 2002; 82: 156–160. Sweeney JD. Standardization of the red cell product. Transfus Apher Sci 2006; 34: 213–218.
21. Zeiler TA, Kretschmer V. Automated blood component collection with the MCS 3p cell separator: evaluation of three protocols for buffy coat – poor and white cell-reduced packed red cells and plasma. Transfusion 1997; 37: 791–797. Sowemimo-Coker SO. Red blood cell hemolysis during processing. Transfus Med Rev 2002; 16: 46–60.
22. Procházková R, Andrýs C, Bláha M, Krejsek J. Storage lesions in red blood cell concentrates from separator Haemonetics MCS+ and Trima Accel. Vox Sanq 2008; 95 (suppl 1): 85.
23. Gyongyosy-Issa MIC, Weiss SL, Sowenimo-Coker SO. Prestorage leukoreduction and low-temperature filtration reduce hemolysis of stored red cell concentrates. Transfusion 2005; 45: 90–96.
24. Hess JR. An update on solutions for red cell storage. Vox Sanq 2006; 91: 13–19. Bandarenko N, Rose M, Kowalsky RJ, et al. In vivo characteristics of double units of RBC collected by apheresis with a single in-line WBC reduction filter. Transfusion 2001; 41: 1373–1377.
25. Sparrow RL, Healey G, Patton KA, Veale FV. Red blood cell age determines the impact of storage and leukocyte burden on cell adhesion molecules, glycophorin A and the release of annexin V. Transfus Apher Sci 2006; 34: 15–23.
26. Högman CF, Löf H, Meryman HT. Storage of red blood cells with improved maintenance of 2,3-bisphosphoglycerate. Transfusion 2006; 46: 1534–1552.
27. Högman CF, Meryman HT. Red blood cells intended for transfusion: quality criteria revisited.Transfusion 2006; 46: 137–142.
28. Offner PJ. Age of blood: does it make a difference? Critical Care 2004; 8 (suppl 2): S24–S26.
29. Blanco L. Tailored collection of multicomponent by apheresis. Transfus Apher Sci 2002; 27: 123–127.
Labels
Haematology Internal medicine Clinical oncologyArticle was published in
Transfusion and Haematology Today
2008 Issue 4
Most read in this issue
- MicroRNA – small molecules with major role (not only) in hematological malignancies
- Imatinib in the first-line treatment of chronic myelogenous leukemia in chronic phase
- Apheresis red cell concentrates - evaluation of apoptosis markers in concentrates obtained from two devices
- Second national study AML-BFM 98 improved remission rate and overall survival in children with acute myeloid leukemia in the Czech Republic