Bioanalytical study of tumour markers for prostate carcinoma at RNA and protein level
Authors:
J. Gumulec 1; M. Masařík 1; S. Křížková 2; P. Babula 2; R. Hrabec 3; A. Rovný 3; M. Masaříková 2; V. Adam 2; T. Eckschlager 4; R. Kizek 2
Authors‘ workplace:
Masarykova univerzita Brno, Lékařská fakulta
Ústav patologické fyziologie
Přednostka: prof. MUDr. Anna Vašků, CSc.
1; Mendelova univerzita v Brně, Agronomická fakulta
Ústav chemie a biochemie
Vedoucí: doc. RNDr. Petr Hrdlička, CSc.
2; Fakultní nemocnice u sv. Anny v Brně
Urologické oddělení
Primář: MUDr. Arne Rovný
3; 2. Lékařská fakulta Univerzita Karlova a Fakultní nemocnice Motol, Praha
Klinika dětské hematologie a onkologie
Přednosta: prof. MUDr. Jan Starý, DrSc.
4
Published in:
Prakt. Lék. 2011; 91(8): 471-476
Category:
Of different specialties
Overview
Current methods for diagnosis of prostate carcinoma do not enable us to distinguish aggressive tumours (significant tumours) from clinically latent tumours (non-significant tumours). This study aims to determine levels of potential clinically important tumour markers such as
- alpha-methyl CoA-racemase (AMACR),
- caveolin-1,
- metallothionein (MT),
- p53,
- NF-κB,
- c-FOS,
- c-JUN,
- Ki-67,
- prostate-specific antigen (PSA),
- ZIP1 and ZnT-1 in prostatic tissue represented by 22Rv1 (tumour) and PNT1A (healthy) cell lines and in blood serum of patients with histologically evaluated adenocarcinoma (82 tumour patients and 51 healthy volunteers).
In serum, the level of MT was significantly enhanced above 1 μM. Caveolin-1 levels were significantly increased in high-grade tumours, which points to the possibility of using this protein as a marker for the aggressive form of prostate carcinoma.
Key words:
tumour diseases, polymerase chain reaction, electrochemistry, immunodetection, molecularly-biological techniques, tumour markers.
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