Molecular biology and oral streptococci
Authors:
M. Krátký
Authors‘ workplace:
Výzkumný ústav stomatologický, Praha
Published in:
Česká stomatologie / Praktické zubní lékařství, ročník , 2000, 2, s. 56-62
Overview
Principles of creation dental plaque, transmission of bacteria inductive dentalcaries or presence of mutant streptococci at infectious endocarditis are the actual researchareas, in which modern molecular-biological methods can be used. The aim of the paper is toclear up the mechanisms of the methods which are applied in identification of bacteria (suchas restriction analysis, sequencing, PCR). Polymerase chain reaction (PCR) is a progressivemethod by which we can amplify the definite part of the nucleotide chain in vitro. Sequencinganalysis of DNA is the most exact (but not the easiest) technique for comparison of congenialityof organisms.
Key words:
streptococci – PCR – RFLP – DNA
Labels
Maxillofacial surgery Orthodontics Dental medicineArticle was published in
Czech Dental Journal
2000 Issue 2
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