Imunohistochemická analýza CD9, CD29 a epitelo-mezenchymové tranzice u triple-negativního karcinomu prsu

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Authors: R.- Ondruššek ^{1 3}; S. Brychtová ¹; M. Bezděková ¹; K. Bouchalova ⁴; Z. Vávrová ⁵; K. Souček ⁶; J. Bouchal ¹
Authors‘ workplace: Department of Clinical and Molecular Pathology, Palacký University and University Hospital Olomouc, Czech Republic ¹; EUC Laboratoře CGB a. s. Ostrava, Czech Republic ²; Department of Molecular and Clinical Pathology and Medical Genetics, University Hospital Ostrava, Czech Republic. ³; Department of Pediatrics, Palacký University and University Hospital Olomouc, Czech Republic ⁴; Department of Surgery, AGEL Hospital Ostrava-Vítkovice, Czech Republic ⁵; Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic, † Deceased ⁶
Published in: Klin Onkol 2024; 38(1): 50-56
Category: Original Articles
doi: https://doi.org/10.48095/ccko202450

Overview

Východiska: Triple-negativní karcinomy prsu (TNBC) jsou heterogenní skupinou nádorů s převážně agresivním chováním a špatnou prognózou. V souvislosti s jejich agresivním chováním a chemorezistencí vůči léčbě se do popředí dostal koncept epitelo-mezenchymové tranzice (EMT). Proteiny CD9 a CD29 jsou spojeny s EMT a mohou hrát roli v progresi TNBC. Naším cílem bylo prozkoumat asociaci těchto markerů s metastázami do lymfatických uzlin, gradingem tumoru, proliferační aktivitou a přežitím pacientů. Pacienti a metody: Náš soubor tvořilo 66 pacientek s TNBC bez neoadjuvantní terapie ve věku 26–81 let. Patologické stadium nádoru se pohybovalo od pT1b do pT3 a histologický stupeň od II do III podle systému Bloom-Richardson. Imunohistochemické hodnocení exprese CD9, CD29, E-cadherinu, vimentinu, androgenového receptoru a Ki-67 bylo provedeno semikvantitativně pomocí H-skóre. Exprese proteinů byla statisticky hodnocena ve vztahu ke klinicko-patologickým parametrům a přežití pacientů. Výsledky: Pozorovali jsme nižší expresi CD9 v metastázách lymfatických uzlin ve srovnání s primárním nádorem (p = 0,021). Exprese CD29 v primárním nádoru byla signifikantně nižší u pacientů s metastázami v lymfatických uzlinách ve srovnání s pacienty bez diseminace (p = 0,03). Ani exprese CD9 ani CD29 proteinu nebyla spojena s přežitím specifickým pro karcinom prsu (BCSS). Nižší exprese E-cadherinu na periferii primárního tumoru byla spojena s horším BCSS (p = 0,038). Pro grading ani přítomnost metastáz v lymfatických uzlinách nebyl nalezen signifikantní vztah s BCSS. Nižší exprese E-cadherinu na periferii byla také spojena s vyšší hladinou Ki67 (Rs −0,26) a vimentinu (Rs −0,33). Závěr: Snížená exprese proteinů CD9 a CD29 byla spojena s růstem metastáz v lymfatických uzlinách, avšak jejich souvislost s přežitím nebyla prokázána. Nižší exprese E-cadherinu na periferii primárního nádoru byla spojena s vysokou proliferací a špatným nádorově specifickým přežitím.

Klíčová slova:

triple-negativní karcinom prsu – CD9 – CD29 – E-cadherin – epitelo-mezenchymová tranzice

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