p‑ SRM, SWATH and HRM – Targeted Proteomics Approaches on TripleTOF 5600+ Mass Spectrometer and Their Applications in Oncology Research

Czech version

Authors: J. Faktor ^1,2; E. Michalová ¹; P. Bouchal ^1,2
Authors‘ workplace: Regionální centrum aplikované molekulární onkologie, Masarykův onkologický ústav, Brno ¹; Ústav biochemie, Přírodovědecká fakulta MU, Brno ²
Published in: Klin Onkol 2014; 27(Supplementum): 110-115

Overview

Development of novel diagnostic and therapeutic approaches in cancer research requires sensitive and quantitative assays for determination of cancer‑associated proteins in clinical samples. Novel quantitative targeted proteomic approaches are overviewed in this communication. A major advantage of selected reaction monitoring (SRM) and pseudo- SRM lies in the selective and sensitive quantification of selected proteins in large sample sets. As such, they represent an alternative to immunochemical approaches. On the other hand, the potential of HRM and SWATH lies in recording of digital fingerprints, which enable post‑acquisition quantitative proteomic data mining on a similar basis to SRM. This article shows applications of targeted proteomics in a number of cancer research studies where they were used for quantification and validation of current or potential protein biomarkers and to study their role in cancer development and progression.

Key words:
proteomics – selected reaction monitoring – oncology – SWATH – biomarkers – molecular diagnostics

This work was supported by the project of Czech Science Foundation No. 14-19250S, by the European Regional Development Fund and the State Budget of the Czech Republic (RECAMO, CZ.1.05/2.1.00/03.0101) and by MH CZ – DRO (MMCI, 00209805) and BBMRI_CZ (LM2010004).

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manuscript met the ICMJE “uniform requirements” for biomedical papers.

Submitted:
3. 2. 2014

Accepted:
26. 3. 2014

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