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Clinical Oncology - Issue Supplementum/2014

7
Programmed Cell Death in Cancer Cells

E. Ondroušková, B. Vojtěšek

15
The Use of Flow Cytometry for Analysis of the Mitochondrial Cell Death

L. Pekarčíková, L. Knopfová, E. Ondroušková, J. Šmarda

22
Methods for Studying Tumor Cell Migration and Invasiveness

P. Kovaříková, E. Michalová, L. Knopfová, P. Bouchal

28
Techniques to Study Transendothelial Migration In Vitro

L. Knopfová, P. Bouchal, J. Šmarda

34
Mechanisms of Drug Resistance and Cancer Stem Cells

J. Holčáková, M. Nekulová, P. Orzol, B. Vojtěšek

42
Functional Assays for Detection of Cancer Stem Cells

J. Škoda, J. Neradil, R. Veselska

48
Tumor Microenvironment –  Possibilities of the Research Under In Vitro Conditions

P. Chlapek, S. Chovanová, V. Sláviková, R. Veselska

53
Electrochemical Analysis of Nucleic Acids, Proteins and Polysaccharides in Biomedicine

M. Bartošík, E. Paleček, B. Vojtěšek

61
Next Generation Sequencing –  Application in Clinical Practice

L. Koubková, B. Vojtěšek, R. Vyzula

69
Development of PCR Methods and Their Applications in Oncological Research and Practice

R. Hrstka, T. Kolářová, E. Michalová, B. Vojtěšek

75
Methods for Analysis of Protein‑protein and Protein‑ligand Interactions

M. Ďurech, F. Trčka, B. Vojtěšek, P. Müller

82
Detection of Protein‑protein Interactions by FRET and BRET Methods

E. Matoulková, B. Vojtěšek

87
In Situ Proximity Ligation Assay for Detection of Proteins, Their Interactions and Modifications

V. Brychtová, B. Vojtěšek

92
Protein Expression and Purification

E. Růčková, P. Müller, B. Vojtěšek

98
Quantitative Mass Spectrometry and Its Utilization in Oncology

L. Hernychová, P. Dvořáková, E. Michalová, B. Vojtěšek

104
Analysis of Protein Using Mass Spectrometry

P. Dvořáková, L. Hernychová, B. Vojtěšek

110
p‑ SRM, SWATH and HRM –  Targeted Proteomics Approaches on TripleTOF 5600+ Mass Spectrometer and Their Applications in Oncology Research

J. Faktor, E. Michalová, P. Bouchal

116
Ananlysis of Phosphoproteins and Signalling Pathwaysby Quantitative Proteomics

M. Pjechová, L. Hernychová, P. Tomašec, G. W. Wilkinson, B. Vojtěšek

121
New Trends in the Study of Protein Glycosylation in Oncological Diseases

M. Zahradníková, L. Hernychová, B. Vojtěšek, M. V. Novotný

129
Current Trends in Using PET Radiopharmaceuticals for Dia­gnostics in Oncology

J. Adam, J. Kadeřávek, F. Kužel, J. Vašina, Z. Řehák

137
„Technetium Crisis“ –  Causes, Possible Solutions and Consequences for Planar Scintigraphy and SPECT Dia­gnostics

J. Adam, J. Kadeřávek, F. Kužel, J. Vašina, Z. Řehák

143
Vitamin D as an Important Steroid Hormone in Breast Cancer

R. Obermannová, R. Demlová, K. Drábová, K. Melichárková, K. Greplová, M. Mrkvicová, L. Zdražilová Dubská, R. Vyzula, D. Valík


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