The cohesin loader SCC2 contains a PHD finger that is required for meiosis in land plants
Autoři:
Hongkuan Wang aff001; Wanyue Xu aff001; Yujin Sun aff003; Qichao Lian aff001; Cong Wang aff001; Chaoyi Yu aff001; Chengpeng He aff001; Jun Wang aff001; Hong Ma aff004; Gregory P. Copenhaver aff003; Yingxiang Wang aff001
Působiště autorů:
State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China
aff001; Center for Epigenetics, Van Andel Institute, Grand Rapids, Michigan, United States of America
aff002; Department of Biology and the Integrative Program for Biological and Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
aff003; Department of Biology, the Huck Institutes of the Life Sciences, the Pennsylvania State University, University Park, Pennsylvania, United States of America
aff004; Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America
aff005
Vyšlo v časopise:
The cohesin loader SCC2 contains a PHD finger that is required for meiosis in land plants. PLoS Genet 16(6): e32767. doi:10.1371/journal.pgen.1008849
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1008849
Souhrn
Cohesin, a multisubunit protein complex, is required for holding sister chromatids together during mitosis and meiosis. The recruitment of cohesin by the sister chromatid cohesion 2/4 (SCC2/4) complex has been extensively studied in Saccharomyces cerevisiae mitosis, but its role in mitosis and meiosis remains poorly understood in multicellular organisms, because complete loss-of-function of either gene causes embryonic lethality. Here, we identified a weak allele of Atscc2 (Atscc2-5) that has only minor defects in vegetative development but exhibits a significant reduction in fertility. Cytological analyses of Atscc2-5 reveal multiple meiotic phenotypes including defects in chromosomal axis formation, meiosis-specific cohesin loading, homolog pairing and synapsis, and AtSPO11-1-dependent double strand break repair. Surprisingly, even though AtSCC2 interacts with AtSCC4 in vitro and in vivo, meiosis-specific knockdown of AtSCC4 expression does not cause any meiotic defect, suggesting that the SCC2-SCC4 complex has divergent roles in mitosis and meiosis. SCC2 homologs from land plants have a unique plant homeodomain (PHD) motif not found in other species. We show that the AtSCC2 PHD domain can bind to the N terminus of histones and is required for meiosis but not mitosis. Taken together, our results provide evidence that unlike SCC2 in other organisms, SCC2 requires a functional PHD domain during meiosis in land plants.
Klíčová slova:
Arabidopsis thaliana – Centromeres – Genetically modified plants – Histones – Meiosis – Mitosis – Pollen – Seeds
Zdroje
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