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The role of flow cytometry in the investigation of lymph node and extranodal lymphatic tissue specimen

Czech version

Authors: Tomáš Zajíc 1;  Jana Včeláková 2;  Vít Campr 2;  Tomáš Jirásek 3
Authors‘ workplace: Oddělení klinické mikrobiologie a imunologie, Centrum laboratorní medicíny, Krajská nemocnice Liberec a. s. 1;  Ústav patologie a molekulární medicíny 2. LF UK a Fakultní nemocnice Motol, Praha 2;  Oddělení patologie, Centrum PATOS, Krajská nemocnice Liberec, a. s. 3
Published in: Čes.-slov. Patol., 59, 2023, No. 4, p. 168-176
Category: Reviews Article

Overview

Although flow cytometry examination of lymphoproliferation in lymph nodes provides minimal morphological information, apart from indicative determination of cell size and granularity, and completely loses topographical information. However, the great advantage of this method is the speed of determining and combination of investigated antigens, where the expression intensities of individual markers are no less important. This is absolutely crucial for hematological malignancies, where different diseases can look very similar at the morphological level and variations in the appearance of tumour cells can be minimal. When diagnosing lymphomas, the combination of histology and immunohistochemistry with flow cytometry provides a more comprehensive view for the interpretation of individual findings. The aim of this article is to provide basic information about the examination of lymph nodes and extranodal tissue by flow cytometry as a starting point for the collaboration of the pathologist and the cytometrist.

Keywords:

Flow cytometry – lymph node – Lymphoproliferative disorder

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Czecho-Slovak Pathology

Issue 4
2023 Issue 4
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