A Comparison of RT-PCR and FISH Techniques in Molecular Diagnosis of Ewing’s Sarcoma in Paraffin-Embedded Tissue
Authors:
A. Berková 1; P. Dundr 2; C. Povýšil 2; Š. Melčáková 2; D. Tvrdík 2
Authors‘ workplace:
Institute of Clinical Biochemistry and Laboratory Diagnostics and 2Institute of Pathology, 1st Faculty of Medicine, Charles University and General Faculty Hospital, Prague, Czech Republic
1
Published in:
Čes.-slov. Patol., 44, 2008, No. 3, p. 67-70
Category:
Original Article
Overview
Ewing’s sarcoma is relatively uncommon tumor representing 6-8 percent of malignant bone tumors with variable morphology. Cytogenetically, Ewing’s sarcomas are characterized by a specific reciprocal chromosomal translocation t(11;22)(q24;q12). The presence of this chromosomal translocation has been detected in approximately 85 percent of the cases. The translocation results in the fusion of EWS gene from chromosome 22 to FLI1 gene at 11q24 which is a member of ETS family of transcription factors. In this study we performed a comparison of two molecular diagnostic strategies, namely RT-PCR and FISH, in fresh, frozen and formalin-fixed paraffin-embedded tissues. We conclude that FISH is a more sensitive technique than RT-PCR for the diagnosis of Ewing’s tumors in formalin-fixed paraffin-embedded tissue. In conclusion, molecular pathology techniques, using reverse transcription-polymerase chain reaction (RT-PCR) and/or fluorescence in situ hybridization (FISH) are valuable diagnostic tools for evaluation of undifferentiated small round-cell tumors like Ewing’s sarcoma.
Key words:
Ewing’s sarcoma – chromosomal translocation – RT-PCR – FISH
Sources
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Czecho-Slovak Pathology
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