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Preimplantation Genetic Diagnosis (PGD) ofChromosomal Aberrations by Fluorescent in situHybridization (FISH) – Introduction to Problem,Sampling Methods and Examination Technique


Authors: K. Veselá;  G. Tauwinklová;  P. Trávník;  J. Veselý;  S. Hlaváčová;  D. Zudová;  O. Řezáčová;  R. Rybář;  J. Rubeš
Authors‘ workplace: Sanatorium REPROMEDA, Brno, vedoucí lékař MUDr. J. Veselý, CSc. 2Výzkumný ústav veterinárního lékařství Brno, ředitel MVDr. M. Toman, CSc. 1
Published in: Ceska Gynekol 2003; (2): 89-94
Category:

Overview

Objective:
Presentation of preimplantation genetic diagnosis and the set of laboratory processes likeaspiration, preparation and evaluation of polar bodies, sperm cells and blastomeres using FISHmethod (fluorescent in situ hybridization) in ART.Design: Review.Setting: Sanatorium REPROMEDA, Brno, Veterinary Research Institute, Brno.Methods: Overview of published data and own clinical experience with the cell aspiration methods,evaluated sample preparation and the proper chromosomes visualisation using FISH method.Conclusion: The review brings an overview of conditions and methods including sample obtaining,FISH analysis preparation and implementation, processed during PGD.

Key words:
preimplantation genetic diagnosis, PGD, FISH, genetic factor, infertility

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Labels
Paediatric gynaecology Gynaecology and obstetrics Reproduction medicine

Article was published in

Czech Gynaecology


2003 Issue 2

Most read in this issue
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