Preimplantation Genetic Diagnosis (PGD) ofChromosomal Aberrations by Fluorescent in situHybridization (FISH) – Introduction to Problem,Sampling Methods and Examination Technique
Authors:
K. Veselá; G. Tauwinklová; P. Trávník; J. Veselý; S. Hlaváčová; D. Zudová; O. Řezáčová; R. Rybář; J. Rubeš
Authors‘ workplace:
Sanatorium REPROMEDA, Brno, vedoucí lékař MUDr. J. Veselý, CSc. 2Výzkumný ústav veterinárního lékařství Brno, ředitel MVDr. M. Toman, CSc.
1
Published in:
Ceska Gynekol 2003; (2): 89-94
Category:
Overview
Objective:
Presentation of preimplantation genetic diagnosis and the set of laboratory processes likeaspiration, preparation and evaluation of polar bodies, sperm cells and blastomeres using FISHmethod (fluorescent in situ hybridization) in ART.Design: Review.Setting: Sanatorium REPROMEDA, Brno, Veterinary Research Institute, Brno.Methods: Overview of published data and own clinical experience with the cell aspiration methods,evaluated sample preparation and the proper chromosomes visualisation using FISH method.Conclusion: The review brings an overview of conditions and methods including sample obtaining,FISH analysis preparation and implementation, processed during PGD.
Key words:
preimplantation genetic diagnosis, PGD, FISH, genetic factor, infertility
Labels
Paediatric gynaecology Gynaecology and obstetrics Reproduction medicineArticle was published in
Czech Gynaecology
2003 Issue 2
Most read in this issue
- Bleeding Pattern in Combined Oral Contraceptive(ethinylestradiol 35 g/norgestimate 250 g) Users:Influence of Regularity of Use and Start Day in theFirst Cycle
- Celiac Disease and Female Infertility
- Does Grapefruit Juice Increase Bioavailabilityof Orally Administered Sexual Steroids?
- Ovum Donation: Ethical and Practical Considerations