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HPLC stanovenie sacharidov po predkolónovej derivatizácií vo vzorkách medu


: Katarína Hroboňová • Jozef Lehotay • Jozef Čižmárik • Pavol Májek • Martin Tuharský
: Institute of Analytical Chemistry, Faculty of Chemical and Food Technology Slovak University of Technology, Bratislava
: Čes. slov. Farm., 2013; 62, 136-143
: Original Articles

Práca je zameraná na hodnotenie kvality vzoriek slovenských medov na základe obsahu minoritných sacharidov. Vzorky zahrňovali jednodruhové a viacdruhové medy. Obsah sacharidov bolo stanovený pomocou HPLC metódy v kombinácií s fluorescenčnou detekciou. Z dôvodu zvýšenia citlivosti detekcie, bola použitá derivatizácia cukrov s dansylhydrazínom. Na HPLC separáciu analytov bola použitá stacionárna fáza typu C18 a zmes acetonitrilu a kyseliny octovej ako mobilná fáza. Vo vzorkách bola zistená prítomnosť sacharózy, maltózy, turanózy a tiež hlavných zložiek glukózy a fruktózy. Medze detekcie boli v rozsahu 15–100 μg.ml–1 a naznačujú, že metóda je vhodná na stanovenie nízkych koncentrácií sacharidov. Pre vzorky slovenských medov a vzorky z iných geografických oblastí (Francúzsko, Chorvátsko) boli zístené rozdiely v obsahu maltózy, turanózy a tiež sacharózy. Pomocou viacrozmernej štatistickej analýzy boli na základe sacharidového profilu slovenské medy rozlíšené od ostatných testovaných medov.

Kľúčové slová:
sacharidy • med • derivatizácia • HPLC


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