HPLC stanovenie sacharidov po predkolónovej derivatizácií vo vzorkách medu
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Katarína Hroboňová • Jozef Lehotay • Jozef Čižmárik • Pavol Májek • Martin Tuharský
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Institute of Analytical Chemistry, Faculty of Chemical and Food Technology Slovak University of Technology, Bratislava
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Čes. slov. Farm., 2013; 62, 136-143
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Original Articles
Práca je zameraná na hodnotenie kvality vzoriek slovenských medov na základe obsahu minoritných sacharidov. Vzorky zahrňovali jednodruhové a viacdruhové medy. Obsah sacharidov bolo stanovený pomocou HPLC metódy v kombinácií s fluorescenčnou detekciou. Z dôvodu zvýšenia citlivosti detekcie, bola použitá derivatizácia cukrov s dansylhydrazínom. Na HPLC separáciu analytov bola použitá stacionárna fáza typu C18 a zmes acetonitrilu a kyseliny octovej ako mobilná fáza. Vo vzorkách bola zistená prítomnosť sacharózy, maltózy, turanózy a tiež hlavných zložiek glukózy a fruktózy. Medze detekcie boli v rozsahu 15–100 μg.ml–1 a naznačujú, že metóda je vhodná na stanovenie nízkych koncentrácií sacharidov. Pre vzorky slovenských medov a vzorky z iných geografických oblastí (Francúzsko, Chorvátsko) boli zístené rozdiely v obsahu maltózy, turanózy a tiež sacharózy. Pomocou viacrozmernej štatistickej analýzy boli na základe sacharidového profilu slovenské medy rozlíšené od ostatných testovaných medov.
Kľúčové slová:
sacharidy • med • derivatizácia • HPLC
Sources
1. Pham P. J., Hernandez R., French W. T., Estill B. G., Mondala A. H. A spectrophotometric method for quantitative determination of xylose in fermentation medium. Biomass and Bioenergy 2011; 35, 2814–2821.
2. Tůma P., Málková K., Samcová E., Štulík K. Rapid monitoring of mono- and disaccharides in drinks, foodstuffs and foodstuff additives by capillary electrophoresis with contactless conductivity detection. Anal. Chim. Acta 2011; 698, 1–5.
3. El Rassi Z., Nashabeh W. Carbohydrate analysis: High performance liquid chromatography and capillary electrophoresis. Amsterdam: Elsevier 1995; 267–360.
4. Caseiro A., Marr I. L., Claeys M., Kasper-Giebl A., Puxbaum H., Pio, C. A. Determination of saccharides in atmospheric aerosol using anion-exchange high-performance liquid chromatography and pulsed-amperometric detection. J. Chromatogr. A 2007; 1171, 37–45.
5. Hemström P., Irgum K. Hydrophilic interaction chromatography. J. Sep. Sci. 2006; 29, 1784–1821.
6. Molnar-Perl I. Role of chromatography in the analysis of sugars, carboxylic acids and amino acids in food. J. Chromatogr. 2000; 91, 1–32.
7. Liang L., Zhang P., Cai Y., Mou, S. High-performance anion exchange chromatography with pulsed amperometric detection for simultaneous determination of monosaccharides and uronic acids. Chinese J. Anal. Chem. 2006; 34, 1371–1374.
8. Takeuchi T., Aspanut Z., Yamada T., Inui A., Lim L. W. Light-scattering detection with a fluorimetric detector in high-performance liquid chromatography. J. Chromatogr. A 2007; 1147, 42–45.
9. Rooyakkers D. R., Van Eijk H. M. H., Deutz N. E. P. Simple and sensitive multi sugar probe gut permeability test using HPLC with fluorescence labelling. J. Chromatogr. A 1996; 730, 99–105.
10. He L., Sato K., Abo M., Okubo A., Yamazaki S. Separation of saccharides derivatized with 2-aminobenzoic acid by capillary electrophoresis and their structural consideration by nuclear magnetic resonance. Anal. Biochem. 2003; 314, 128–134.
11. Zhang P., Wang Z., Xie M., Nie W., Huang L. Detection of carbohydrates using a pre-column derivatization reagent 1-(4-isopropyl) phenyl-3-methyl-5-pyrazolone by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry. J. Chromatogr. B 2010; 878, 1135–1144.
12. Yamamoto K., Hamase K., Zaitsu K. 2-Amino-3-phenylpyrazine, a sensitive fluorescence prelabeling reagent for the chromatographic or electrophoretic determination of saccharides. J. Chromatogr. A 2003; 1004, 99–106.
13. Lattova E., Perreault H. Labelling saccharides with phenylhydrazine for electrospray and matrix-assisted laser desorption-ionization mass spectrometry. J. Chromatogr. B 2003; 793, 167–179.
14. Todoroki K., Hayama T., Ijiri S., Kazuta A., Yoshida H., Nohta H., Yamaguchi M. Rhodamine B amine as a highly sensitive fluorescence derivatization reagent for saccharides in reversed-phase liquid chromatography. J. Chromatogr. A 2004; 1038, 113–120.
15. Kakita H., Kamishima H., Komiya K., Kato Y. Simultaneous analysis of monosaccharides and oligosaccharides by high-performance liquid chromatography with post-column fluorescence derivatization. J. Chromatogr. A 2002; 961, 77–82.
16. Mopper K., Jonhnson L. Reversed-phase liquid chromatographic analysis of Dns-sugars. Optimization of derivatization and chromatographic procedures and applications to natural samples. J. Chromatogr. 1983; 256, 27–38.
17. Hroboňová K., Lehotay J., Jablonský M., Katuščák S. HPLC determination of saccharides in the process of accelerated ageing of paper. Chemické listy 2009; 103, 744–751.
18. White J. W. Honey. Advances in food research 1978; 24, 287–374.
19. Otto M. Chemometrics, 2nd Edition. New York: Wiley-VCH 2007.
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Pharmacy Clinical pharmacologyArticle was published in
Czech and Slovak Pharmacy
2013 Issue 3
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