Droplet digitálna PCR ako nový diagnostický nástroj

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Authors: B. Váňová ^1,2; B. Malicherova ²; T. Burjanivová ³; A. Liskova ⁴; K. Janikova ^2,5; K. Jasek ²; Z. Lasabová ³; M. Tatár ⁵; L. Plank ⁶
Authors‘ workplace: Martin´s Center of Immunology, Ltd., Martin, Slovakia ¹; Biomedical Center Martin JFM CU, Martin, Slovakia ²; Department of Molecular Biology JFM CU, Martin, Slovakia ³; Clinic of Obstetrics and Gynecology JFM CU and University Hospital in Martin, Slovakia ⁴; Department of Pathological Physiology JFM CU, Martin, Slovakia ⁵; Department of Pathological Anatomy, JFM CU and University Hospital in Martin, Slovakia ⁶
Published in: Klin Onkol 2021; 34(1): 33-39
Category: Review
doi: https://doi.org/10.48095/ccko202133

Overview

Východiská: Podstatou moderných postupov liečby onkologických pacientov je v dnešnej dobe zacielenie konkrétnych molekúl zapojených do bunkovej signalizácie asociovanej s nádorovou iniciáciou a progresiou. Úspech uvedeného prístupu závisí od správne zvoleného diagnostického testu s vysokou citlivosťou, ktorý identifikuje výskyt a hladinu vybraných biomarkerov u pacientov pre selekciu tých, ktorí budú na liečivo reagovať a budú z neho benefitovať. Vývoj nových technológií a modernizácia tých známych, prispievajú k inováciám molekulárnej charakterizácie karcinómov, ktorá umožňuje detekciu mutačného stavu pacienta s vysokou citlivosťou a špecifickosťou. Cieľ: V práci diskutujeme o využití polymerázovej reťazovej reakcie (PCR) tretej generácie, tzv. droplet digitálnej PCR (ddPCR), v molekulárnej diagnostike karcinómov. Podľa štúdií uvedených v našom prehľade predstavuje ddPCR sľubný nástroj pri vytváraní genetického profilu pacientov s onkologickým ochorením. Optimalizácia a presná validácia môžu preto umožniť postupnú implementáciu ddPCR do klinickej praxe v oblasti onkológie.

Klíčová slova:

rakovina – nádorové biomarkery – molekulárna diagnostika – ddPCR

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