Relations between Selected Cytokines, Adhesive Immunoglobulinsand Matrix Metalloproteinases in Osteoarthritic Joints

Overview

Background.
The disbalance of proteinase production and their inhibitors leads to destruction of tissues in manypathological processes. Recently it was shown that the expression of proteinases is regulated also by interactions ofcells which is mediated by adhesive molecules. We wanted to find out whether this mechanism is involved also inthe destruction of joints in osteoarthritis.Methods and Results. Cartilage, synovial and subchondral bone tissues, and synovial fluids were obtained from23 joints after total endoprosthesis surgery. The solid tissues were extracted by TRIS buffer. The investigated proteinconcentrations were assessed imunochemicaly. In all specimens gelatinase A, gelatinase B, stromelysin-1, TIMP-1,soluble adhesive molecules sICAM-1 and sVCAM-1 and cytokines TNF-α and IL-8 were found. In cartilage andsynovial fluid the proteolytic potential of metalloproteinases was balanced with high concentrations of their inhibitorTIMP-1 (259.4±105.2 pmol/g protein vs 2343.8±637.5 pmol/g protein in synovial fluid, p

Key words:
osteoarthritis, adhesive molecules, cytokines, matrix metalloproteinases.