Microglial activation and GFAP astroglial immunopositivity in drug abusers

Czech version

Authors: D. Vajtr ¹; J. Kukačka ²; P. Strejc ¹; A. Vitouš ³; M. Balíková ¹; E. Bradková ¹; A. Pilin ¹; R. Průša ²
Authors‘ workplace: Ústav soudního lékařství a toxikologie VFN a 1. LF UK, Praha ¹; Ústav klinické biochemie a patobiochemie FN Motol a 2. LF UK, Praha ²; Klinika infekčních nemocí FN Motol a 2. LF UK, Praha ³
Published in: Soud Lék., 57, 2012, No. 2, p. 31-33
Category: Original Article

Overview

Introduction:
The neurotoxicity brought about by application of toxic and psychotropic substances is accompanied by an activation of astroglial and microglial cells in the brain.

Materials and method:
We investigated clinically 42 patients addicted to psychotropic substances (hospitalised in the Motol Teaching Hospital). The NSE, S1OOB, and manganese concentrations in the blood were measured in the patients. In 14 deceased patients with drug evidence in the hair indicating a chronic abuse of addictive substances, the brain tissue glial cells were immunohistochemically labelled with antibody against CD68 and GFAP.

Results:
In 8 hospitalised patients, there were increased NSE, S100B values in the blood (p < 0.05). Manganese in the blood was increased (3,03±1,9 μg/l, p < 0,05) in all patients. In deceased persons, the CD68 positivity of microglial cells and neurophagy have been proved. GFAP positive astroglial cells have been evidenced in the gray and white matter.

Conclusion:
The CD68 and GFAP positive glial cells in brain tissue can be a pathomorphological correlate of neurotoxicity in chronic abusers. The neurotoxicity can be monitored with NSE and S100B markers of damaged neuronal cells.

Keywords:
neurotoxicity – mikroglial cells – astroglial cells – neuron specific enolasis – S-100B protein

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Labels

Anatomical pathology Forensic medical examiner Toxicology

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Forensic Medicine

2012 Issue 2