#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Nonculture techniques of microorganisms determination in amniotic fluid in patients with preterm prelabor rupture of membranes


Authors: R. Kutová 1;  L. Plíšková 1;  M. Kacerovský 2
Authors‘ workplace: Ústav klinické biochemie a diagnostiky FN, Hradec Králové, přednostka MUDr. L. Pavlíková 1;  Porodnická a gynekologická klinika FN, Hradec Králové, přednosta doc. MUDr. J. Špaček, Ph. D., IFEPAG 2
Published in: Ceska Gynekol 2016; 81(5): 384-388

Overview

Objective:
The preterm prelabor rupture of membranes is a serious obstetric complication that is frequently complicated by the presence of microorganisms in amniotic fluid. The aim of our work is to characterize current status of nonculture detection of microbial invasion into the amniotic cavity and the experience with the technique performed in University Hospital in Hradec Kralove.

Design:
Original survey article.

Setting:
Institute of Clinical Biochemistry and Diagnostics – molecular biology department, University Hospital Hradec Kralove.

Conclusion:
Application of nonculture techniques of microorganisms determination in amniotic fluid in patients with preterm prelabor rupture of membranes is currently available. According to the detection of genital mycoplasmas as the dominant pathogens in the amniotic fluid this technique should be regarded as the standard examination method in these patients.

Keywords:
preterm labor, genital mycoplasmas, polymerase chain reaction, bacterial DNA


Sources

1. Bredeson, S., Papaconstantinou, J., Difford, JH., et al. HMGB1 promotes a p38MAPK associated non-infectious inflammatory response pathway in human fetal membranes. PLoS One, 2014, 9, e113799. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0113799

2. Combs, CA., Gravett, M., Garite, TJ., et al. Amniotic fluid infection, inflammation, and colonization in preterm labor with intact membranes. Am J Obstet Gynecol, 2014, 210, p. 121–125. http://www.ncbi.nlm.nih.gov/pubmed/24274987

3. DiGiulio, DB., Romero, R., Amogan, HP., et al. Microbial prevalence, diversity and abundance in amniotic fluid during preterm labor: a molecular and culture-based investigation. PLoS ONE, 2008, 3(8): e3056. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0003056

4. DiGiulio, DB., Romero, R., Kusanovic, JP., et al. Prevalence and diversity of microbes in the amniotic fluid, the fetal inflammatory response, and pregnancy outcome in women with preterm pre-labor rupture of membranes. Am J Reprod Immunol, 2010, 64, p. 38–57. http://www.ncbi.nlm.nih.gov/pubmed/20331587

5. Fouhy, F., Deane, J., Rea, MC., et al. The effects of freezing on faecal microbiota as determined using MiSeq sequencing and culture-based investigations. PLoS ONE, 2015, 10(3), e0119355. http://www.ncbi.nlm.nih.gov/pubmed/25748176

6. Goldenberg, RL., Culhane, JF., Iams, JD., Romero, R. Epidemiology and causes of preterm birth. Lancet, 2008, 371, p.75–84. http://www.ncbi.nlm.nih.gov/pubmed/18177778

7. Gregory, KE. Microbiome aspects of perinatal and neonatal health. J. Perinat. Neonatal Nurs, 2011, 25, p. 158–164. http://www.ncbi.nlm.nih.gov/pubmed/21540692

8. Chakravorty, S., Helb, D., Burday, M., et al. A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria. J Microbiol Methods, 2007, 69, p. 330–339. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2562909/

9. Chaemsaithong, P., Romero, R., Korzeniewski, SJ., et al. A point of care test for the determination of amniotic fluid interleukin-6 and the chemokine CXCL-10/IP-10. J Matern Fetal Neonatal Med, 2015, 28(13), p. 1510–1519. http://www.ncbi.nlm.nih.gov/pubmed/25182862

10. Chaemsaithong, P., Romero, R., Korzeniewski, SJ., et al. A point of care test for interleukin-6 in amniotic fluid in preterm prelabor rupture of membranes: a step toward the early treatment of acute intra-amniotic inflammation/infection. J Matern Fetal Neonatal Med, 2016, 29(3), p. 360–367. http://www.ncbi.nlm.nih.gov/pubmed/25758620

11. Chaemsaithong, P., Romero, R., Korzeniewski, SJ., et al. A rapid interleukin-6 bedside test for the identification of intra-amniotic inflammation in preterm labor with intact membranes. J Matern Fetal Neonatal Med, 2016, 29(3), p. 349–359. http://www.ncbi.nlm.nih.gov/pubmed/25758618

12. Jacobsson, B., Mattsby-Baltzer, I., Andersch, B., et al. Microbial invasion and cytokine response in amniotic fluid in a Swedish population of women with preterm prelabor rupture of membranes. Acta Obstet Gynecol Scand, 2003, 82, p. 423–431. http://www.ncbi.nlm.nih.gov/pubmed/12752072

13. Jacobsson, B., Aaltonen, R., Rantakokko-Jalava, K., et al. Quantification of Ureaplasma urealyticum DNA in the amniotic fluid from patients in PTL and pPROM and its relation to inflammatory cytokine levels. Acta Obstet Gynecol Scand, 2009, 88, p. 63–70. http://www.ncbi.nlm.nih.gov/pubmed/19031281

14. Kacerovsky, M., Pliskova, L., Bolehovska, R., et al. The microbial load with genital mycoplasmas correlates with the degree of histologic chorioamnionitis in preterm PROM. Am J Obstet Gynecol, 2011, 205, p. 213 e211–217. http://www.ncbi.nlm.nih.gov/pubmed/21663889

15. Kacerovsky, M., Musilova, I., Khatibi, A., et al. Intraamniotic inflammatory response to bacteria: analysis of multiple amniotic fluid proteins in women with preterm prelabor rupture of membranes. J Matern Fetal Neonatal Med, 2012, 25, p. 2014–2019. http://www.tandfonline.com/doi/abs/10.3109/14767058.2012.671873?journalCode=ijmf20#.V1KjQOTKDIU

16. Kacerovsky, M., Pliskova, L., Bolehovska, R., et al. The impact of the microbial load of genital mycoplasmas and gestational age on the intensity of intraamniotic inflammation. Am J Obstet Gynecol, 2012, 206, p. 342 e341–348. http://www.ncbi.nlm.nih.gov/pubmed/22340945

17. Kacerovsky, M., Celec, P., Vlkova, B., et al. Amniotic fluid protein profiles of intraamniotic inflammatory response to Ureaplasma spp. and other bacteria. PLoS One, 2013, 8, e60399. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0060399

18. Menon, R., Boldogh, I., Hawkins, HK., et al. Histological evidence of oxidative stress and premature senescence in preterm premature rupture of the human fetal membranes recapitulated in vitro. Am J Pathol, 2014, 184, p. 1740–1751. http://www.ncbi.nlm.nih.gov/pubmed/24832021

19. Menon, R., Polettini, J., Syed, TA., et al. Expression of 8-oxoguanine glycosylase in human fetal membranes. Am J Reprod Immunol, 2014, 72, p. 75–84. http://www.ncbi.nlm.nih.gov/pubmed/24589083

20. Musilova, I., Kutova, R., Pliskova, L., et al. Intraamniotic inflammation in women with preterm prelabor rupture of membranes. PloS ONE, 2015, 10(7), e0133929. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133929

21. Petrosino, JF., Highlander, S., Luna, RA., et al. Metagenomic pyrosequencing and microbial identification. Clin Chem, 2009, 55, p. 856–866. http://www.ncbi.nlm.nih.gov/pubmed/19264858

22. Příbalová informace kitu AmpliSens© C. trachomatis/Ureplasma/M. hominis/M. genitalium – MULTIPRIME-FRT firmy AmpliSens.

23. Romero, R., Ghidini, A., Mazor, M., Behnke, E. Microbial invasion of the amniotic cavity in premature rupture of membranes. Clin Obstet Gynecol, 1991, 34, p. 769–778.

24. Romero, R., Yoon, BH., Kendy, JS., et al. Amniotic fluid interleukin-6 determinations are of diagnostic and prognostic value in preterm labor. Am J Reprod Immunol, 1993, 30, p. 167–183. http://www.ncbi.nlm.nih.gov/pubmed/8311926

25. Romero, R., Yoon, BH., Mazor, M., et al. A comparative study of the diagnostic performance of amniotic fluid glucose, white blood cell count, interleukin-6, and gram stain in the detection of microbial invasion in patients with preterm premature rupture of membranes. Am J Obstet Gynecol, 1993, 169, p. 839–851. http://www.ncbi.nlm.nih.gov/pubmed/7694463

26. Romero, R., Espinoza, J., Goncalves, LF., et al. The role of inflammation and infection in preterm birth. Semin Reprod Med, 2007, 25, p. 21–39. http://www.ncbi.nlm.nih.gov/pubmed/17205421

27. Romero, R., Miranda, J., Chaemsaithong, P., et al. Sterile and microbial-associated intra-amniotic inflammation in preterm prelabor rupture of membranes. J Matern Fetal Neonatal Med, 2014, 28 (12), p. 1394–409. http://www.ncbi.nlm.nih.gov/pubmed/25190175

28. Romero, R., Kadar, N., Miranda, J., et al. The diagnostic performance of the Mass Restricted (MR) score in the identification of microbial invasion of the amniotic cavity or intra-amniotic inflammation is not superior to amniotic fluid interleukin-6. J Matern Fetal Neonatal Med, 2014, 27, p. 757–769. http://www.ncbi.nlm.nih.gov/pubmed/24028673

29, Romero, R., Hassan, SS., Gajer, P., et al. The composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women. Microbiome, 2014, 2(4). http://www.ncbi.nlm.nih.gov/pubmed/24484853

30. http://www.alimetrics.net/en/index.php/dna-sequence-analysis

Labels
Paediatric gynaecology Gynaecology and obstetrics Reproduction medicine
Topics Journals
Login
Forgotten password

Enter the email address that you registered with. We will send you instructions on how to set a new password.

Login

Don‘t have an account?  Create new account

#ADS_BOTTOM_SCRIPTS#