Inactivation of BRCA1, BRCA2 and p53 Genes in Sporadic Ovarian Cancer
Authors:
Michal Zikán 1; P. Pohlreich 2; P. Freitag 1; M. Janoušek 1; D. Pavlišta 1; D. Fischerová 1; N. Jančárková 1; J. Sláma 1; I. Pinkavová 1; D. Cibula 1
Authors‘ workplace:
Onkogynekologické centrum, Gynekologicko-porodnická klinika 1. LF UK a VFN, Praha, přednosta prof. MUDr. A. Martan, DrSc.
1; Ústav biochemie a experimentální onkologie 1. LF UK, přednosta prof. MUDr. A. Šedo, DrSc.
2
Published in:
Ceska Gynekol 2008; 73(5): 298-302
Category:
Original Article
Overview
Objective:
To analyze loss of heterozygosity (LOH), loss of expression and somatic mutations of BRCA1, BRCA2 and p53 genes in sporadic epithelial ovarian cancer samples.
Design:
Original paper.
Setting:
Oncogynecologic center, Clinic of Obstetrics and gynecology, First Faculty of Medicine, Charles University in Prague and General Teaching Hospital, Prague.
Material and methods:
We used genomic DNA and total RNA from peripheral blood and fresh frozen tumor as a template for LOH, loss-of-expression and mutation analyses.
Results:
LOH in at least one region was found in 60% of tumors. Majority of these alterations occurred not solely, but in conjunction with other region deletions.
Conclusion:
Our study confirms high frequency of somatic alteration of BRCA1, BRCA2 and p53 genes in sporadic epithelial ovarian cancer.
Key words:
BRCA1, BRCA2, p53, ovarian cancer, LOH.
Sources
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Labels
Paediatric gynaecology Gynaecology and obstetrics Reproduction medicineArticle was published in
Czech Gynaecology
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