The Effect of Jasminic Acid on the Production of Anthracene Derivatives in the Cultureof Rheum palmatum L. in vitro

Overview

The process of elicitation makes use of the capacity of plants and plant cells cultivated in vitro toreact to various stress stimuli by a number of protective reactions leading to increased accumulationof secondary metabolites. The endogenic signal substances of plant protective reactions includejasminic acid, which in the case of exogenous application also acts as an elicitor. The paper examinedthe effect of four concentrations of jasminic acid on the production of anthracene derivatives bya three-year-old tissue culture, which was derived from the roots of a two-year-old intact plantRheum palmatum L. (Polygonaceae). The culture was cultivated on Murashige-Skoog medium withan addition of 10 mg.l-1 of α-naphthylacetic acid. The results show that the optimal effect of jasminicacid on the production of callus culture was manifested after a 12-hour application of the strongestconcentration of 5 mM, and on the production of suspension culture after a 48-hour application ofa concentration of 0.05mM,when the photometric determination according to PhBs 4 demonstratedthe maximal content of anthracene derivatives (1.26 %) and the productionwas stimulated by 109 %in comparison with the control.

Key words:
Rheum palmatum L. in vitro – anthracene derivatives – elicitation – jasminic acid