Assessment of methylated derivatives of glycine in patients with metabolic syndrome and prediabetes
Authors:
V. Sudová; J. Racek; D. Rajdl; L. Trefil
Authors‘ workplace:
Ústav klinické biochemie a hematologie, Univerzita Karlova v Praze - Lékařská fakulta v Plzni a Fakultní nemocnice Plzeň
Published in:
Klin. Biochem. Metab., 27, 2019, No. 3, p. 107-111
Overview
Objective: The aim of our study was the implementation of HPLC method with UV detection for betaine and dimethylglycine in human plasma samples. Then quantification of these analytes in patients with metabolic syndrome and prediabetes and compare results between them and the control group.
Design: retrospective.
Settings: Department of Clinical Biochemistry and Hematology, Faculty of Medicine, Charles University and Faculty Hospital, Alej Svobody 80, 304 60 Pilsen (Czech Republic).
Material and methods: We developed the HPLC method on liquid chromatograph Thermo Separation Products Spectra SYSTEM (Thermo Fisher Scientific, Waltham, MA, USA) with these chromatographic conditions: mobile phase consisting of 90 % acetonitrile, 10 % water and 22 mmol/L choline; flow rate 1.5 ml/min; temperature 33 °C; UV detection in the wavelength 254 nm; column SUPELCOSILTM LC-SCX with particle size 5 µm. For implementation of method we used plasma samples of blood donors from transfusion department Faculty Hospital in Pilsen. The group consisted of 15 males and 15 females in the range of age of 39 to 61 years. The group with metabolic syndrome and prediabetes contained 12 males and 8 females in the range of age 36 to 67 years.
Results: Values of precision expressed as coefficient of variation were 10.6 % and 11.8 % for betaine and dimethylglycine. Intermediate precisions were 7.1 % and 12.3 %. Recovery of both analytes were 97.8 % for betaine and 97.7 % for dimethylglycine. Plasmatic concentrations of betaine and dimethylglycine in the control group were 135.8 ± 47.4 µmol/L and 4.7 ± 1.3 µmol/L. Plasmatic concentrations in patients with metabolic syndrome and prediabetes were 100.9 ± 40.9 µmol/L (significantly lower than in control group, p < 0.005) and 10.3 ± 6.2 µmol/L (significantly higher than in control group, p < 0.01). Plasma concentration of homocysteine in patients with metabolic syndrome was 14.4 ± 5.0 µmol/L (p < 0.001), in the control group 10.0 ± 3.5 µmol/L.
Conclusion: Patients with metabolic syndrome and prediabetes had lower plasma concentration of betaine and higher plasma level of dimethylglycine and homocysteine. These changes could be in relation with the development of non-alcoholic fatty liver disease and increased cardiovascular risk in these patients.
Keywords:
metabolic syndrome – betaine – dimethylglycine – HPLC.
Sources
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Clinical biochemistry Nuclear medicine Nutritive therapistArticle was published in
Clinical Biochemistry and Metabolism
2019 Issue 3
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