Výskyt Mycoplasma hominis a  Ureaplasma urealyticum u žen s poruchou fertility

English version Czech version

: R. Sleha ^1,2; V. Boštíková ¹; R. Hampl ³; M. Salavec ⁴; P. Halada ⁵; Martin Štěpán ⁵; Š. Novotná ³; R. Kukla ²; E. Slehová ²; M. Kacerovský ⁵; P. Boštík ⁶
: University of Defence, Faculty of Military Health Sciences, Department of Epidemiology, Hradec Kralove, Czech Republic ¹; University of Pardubice, Faculty of Chemical-Technology, Department of Biology and Biochemistry, Pardubice, Czech Republic ²; The Centre of Assisted Reproduction SANUS, Pardubice, Czech Republic ³; Department of Dermatovenereology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic ⁴; University Hospital, Department of Obstetrics and Gyneacology, Hradec Kralove, Czech Republic ⁵; University of Defence, Faculty of Military Health Sciences, The Centre of Advanced Studies, Hradec Kralove, Czech Republic ⁶
: Epidemiol. Mikrobiol. Imunol. 65, 2016, č. 4, s. 232-237
: Original Papers

Cíle:
Mycoplasma hominis a Ureaplasma urealyticum jsou podmíněně patogenní mikroorganismy, které se frekventovaně vyskytují v urogenitálním ústrojí žen. Uvedené bakteriální druhy mohou způsobovat infekce urogenitálního traktu a jsou rovněž spojovány s nepříznivým vlivem na průběh těhotenství, případně s lidskou neplodností. Cílem této práce bylo zjistit četnost výskytu M. hominis a U. urealyticum u žen z asistované reprodukce ve srovnání s kontrolní skupinou plodných žen.

Metody:
Průkaz mykoplazmat v cervikálních stěrech byl prováděn kultivačním vyšetřením a polymerázovou řetězovou reakcí. U izolovaných kmenů byla následně ověřena mikrodiluční bujónovou metodou jejich citlivost k azithromycinu, ciprofloxacinu, doxycyklinu a erythromycinu.

Výsledky:
Celkově bylo vyšetřeno 111 žen s poruchou reprodukční funkce. U. urealyticum bylo prokázáno ve stěrech 44 žen (39,6 %). Výskyt M. hominis byl zaznamenán pouze u 9 žen (8,1 %). Z tohoto počtu bylo 6 žen (5,4 %) pozitivních na přítomnost obou bakteriálních druhů. Kontrolní skupinu tvořilo 23 žen. Přítomnost U. urealyticum byla detekována u 8 žen (34,7 %). M. hominis bylo detekováno pouze ve směsi s U. urealyticum, a to ve 3 případech (13,0 %). Nejvyšší inhibiční aktivita na oba testované druhy byla pozorována u doxycyklinu.

Závěr:
Výsledky ukazují na vyšší incidenci M. hominis a U. urealyticum v genitálním traktu žen s problémy v reprodukci ve srovnání s kontrolní skupinou. Potenciální negativní efekt na reprodukční schopnost žen nebyl pozorován.

KLÍČOVÁ SLOVA:
mykoplazmata – ureaplazmata – ženy – neplodnost – asistovaná reprodukce

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