Medium with Amikacin for Laboratory Diagnosis of Beta-haemolytic Streptococci

Overview

Beta-haemolytic streptococci are implicated in the etiology of secondary wound infection, ulcerations and decubitus ulcers. Laboratory diagnosis of these causative agents is complicated by the fact that other less fastidious bacteria (Staphylococcus aureus, pseudomonads and enterobacteria) may also be involved in the infection. On blood agar, these bacteria overgrow colonies of beta-haemolytic streptococci.

Detectability of beta-haemolytic streptococci on blood agar and selective blood agar added with amikacin (36 μg/ ml) was compared.

A total of 836 specimens from 782 patients were examined. Eighty-six isolates of beta-haemolytic streptococci were detected.

Coinfection with other bacterial species, most frequently identified as Staphylococcus aureus and Pseudomonas aeruginosa, was observed in 73 specimens. Forty-six isolates of beta-hemolytic streptococci involved in coinfection were detected on both blood agar and blood agar with amikacin.

Twenty-seven additional isolates were only recovered on blood agar with amikacin. Without the use of selective medium, 37 % of isolates of beta-haemolytic streptococci from skin lesion specimens would escape detection.

Key words:
beta-haemolytic streptococci – secondary skin infection – selective medium.