HPV Infection and CervicalIntraepithelial Hyperplasia

Overview

Objective:
To determine the presence of high-risk, oncogenic types of HPV infection (HR HPV) andthe presence of HPV 16 and 18 in various degrees of cervical and intraepithelial neoplasia.Type of the study: A retrospective study.Name and Address of the Institution: Obstetrical-Gynecological Clinic, Faculty Hospital, Ostrava.Methods: The study included 205 female patients indicated for conization due to cervical intraepithelialneoplasia. The conization was preceded by extended colposcopy, cytological samplings, HPVDNA test and a directed biopsy. HR HPV was examined in all patients, HPV 18 and 16, respectively,in 98 (48%) patients. Based on severity of histopathological findings the patients were divided intothree groups, examined for positivity of oncogenic HPV types and the high risk HPV 18 and HPV18 types, respectively.Results: The histopathological findings froma total number of 205 conuses included five (2%) benignlesions, 85 (41%) CIN1, 77 (38%) CIN 2 and 38 (19%) CIN 3. Altogether the HR HPV was demonstratedin 143 (70%) patients. In patients with the benign lesion no HPV infection was detected. In patientswith the CIN 1 finding the HR HPV infection was detected in 35 (41%) cases, in patients with CIN 2in 70 (91%) cases and in pacients with CIN 3 in 38 (100%) cases. HPV 16 was diagnosed in patientswith the CIN 1 finding in 9 (23%) cases, HPV 16 in 5 (6%) cases and both types of the virus werepresent in two (2%) cases. In CIN 2, HPV 16 was positive in 17 (43%) and HPV 18 in 6 (8%) cases. Inpatients with CIN 3, HPV 18 was detected in 9 (53%) and HPV 18 in 2 (5%) patients. In all the womenwho underwent conization, HPV 16 infection was demonstrated in 17% and HPV 18 in 6% pacients.In 19% of patients, the infection by at least one type of the virus was present and in two % both typesof the virus were present. Conclusion: the presence of HR HPV rises with increasing severity of cervical intraepithelialneoplasia, HR HPV being detected in all cases suffering from CIN 3. The highest risk type HPV 16was present with increaing rate in relation to the severity of cervical intraepithelial neoplasia,whereas no such trend was present in case of HPV 18.

Key words:
papillomavirus, cervical intraepithelial neoplasia, HPV test