Immunofluorescence analysis of proapoptotic signaling molecules in human melanoma cells after photodynamic treatment
Authors:
Robert Bajgar 1; Hana Kolářová 1; Svatopluk Binder 1; Adéla Dašková 1,2; Hana Lenobelová 1; Klára Pížová 1,2; Kateřina Tománková 1
Authors‘ workplace:
Ústav lékařské biofyziky, Lékařská fakulta, Univerzita Palackého, Olomouc
1; Ústav molekulární a translační medicíny, Lékařská fakulta, Univerzita Palackého, Olomouc
2
Published in:
Lékař a technika - Clinician and Technology No. 1, 2013, 43, 15-18
Category:
Original research
Overview
Photodynamic therapy (PDT) is connected with oxidative damage of biomolecules causing significant impairment of essential cellular functions that lead to cell death. It is the reason, why photodynamic therapy has also found its application in treatment of different oncological, cardiovascular, skin and eye diseases. The cell death after PDT is mediated by an apoptotic and/or necrotic process including activation of various biomolecules.
In the presented study we have used immunofluorescence method to detect caspase 3 and 9, poly ADP-ribose polymerase (PARP) in their active forms, and release of the cytochrome c as the proapoptotic protein after photodynamic treatment of human melanoma cells.
Keywords:
apoptosis, photodynamic therapy, enzyme-linked immunosorbent assay (ELISA)
Sources
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