Use of platelet lysate for mesenchymal stem cell cultivation

Czech version

Authors: E. Matějková ¹; V. Foltánková ²; J. Michálek ²; B. Kubešová ¹
Authors‘ workplace: Tkáňová banka FN Brno, pracoviště Bohunice, přednostka prim. MUDr. Barbara Kubešová ¹; Univerzitní centrum buněčné imunoterapie, Babákův výzkumný institut Lékařské fakulty MU Brno, přednosta prof. MUDr. Roman Hájek, CSc. ²
Published in: Vnitř Lék 2010; 56(Supplementum 1): 46-50
Category: 16th Parizek's Days, Ostrava-Poruba, March 25th –26th 2010

Overview

Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells that can be isolated from many adult tissues. They can differentiate into cells of the mesodermal lineage, such as adipocytes, osteocytes and chondrocytes, as well as cells of other embryonic lineages. MSCs can interact with cells of both the innate and adaptive immune systems, leading to the modulation of several effector functions. After in vivo administration, MSCs induce peripheral tolerance and migrate to injured tissues, where they can inhibit the release of pro-inflammatory cytokines and promote the survival of damaged cells. This article recapitulates experimental data of MSC sources and mechanisms of MSC- isolation and cultivation according to GMP (good manufacture practice) standards.

Key words:
mesenchymal stem cells – platelet lysate

Sources

1. Friedenstein AJ, Petrakova KV, Kurolesova AI et al. Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation 1968; 6: 230– 247.

2. Friedenstein AJ. Precursor cells of mechanocytes. Int Rev Cytol 1976; 47: 327– 359.

3. Friedenstein AJ, Chailakhyan RK, Gerasimov UV. Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. Cell Tissue Kinet 1987; 20: 263– 272.

4. Horwitz EM, Le Blanc K, Dominici M et al. Clarification of the nomenclature for MSC: The International Society for Cellular Therapy position statement. Cytotherapy 2005; 7: 393– 395.

5. Kern S, Eichler H, Stoeve J. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells 2006; 5: 1294– 1301.

6. Wagner W, Wein F, Seckinger A et al.Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood. Exp Hematol 2005; 11: 1402– 1416.

7. Panepucci RA, Siufi JL, Silva jr. WA. Comparison of gene expression of umbilical cord vein and bone marrow- derived mesenchymal stem cells. Stem Cells 2004; 22: 1263– 1278.