#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Flow cytometry in analysis of platelets


Authors: L. Kovářová
Authors‘ workplace: Oddělení klinické hematologie FN Brno, pracoviště Bohunice, přednosta prof. MUDr. Miroslav Penka, CSc.
Published in: Vnitř Lék 2010; 56(Supplementum 1): 117-119
Category: 15th Parizek's Days

Overview

Summary:
Flow cytometry is a modern screening method used for cell analysis in many clinical and research laboratories. In the study of biology and functional status of platelets are its applications: 1. detection of inherited and acquired platelets defects; 2. platelets activation in vivo; 3. applications in blood transfusion; 4. platelet turnover studies. Each of these applications has its advantages and drawbacks. The aim of this paper is to summarize and explain basic applications of flow cytometry and its use in analysis of platelets.

Key words:
flow cytometry – platelets


Sources

1. Brown M, Wittwer C. Flow cytometry: principles and clinical applications in hematology. Clin Chem 2000; 46: 1221–1229.

2. Gibbins JM, Mahaut-Smith MP. Platelets and Megakaryocytes. New Jersey: Humana Press 2004; 1. vol.: Functional Assays: 225–253.

3. Lindahl TL, Ramström S. Methods for evaluation of platelet function. Transfus Apher Sci 2009; 41: 121–125.

4. Nurden AT. Glanzmann thrombasthenia. Orphanet J Rare Dis 2006; 1: 10.

5. Beltrame MP, Malvezzi M, Zanis J et al. Flow cytometry as a tool in the diagnosis of Bernard-Soulier syndrome in Brazilian patients. Platelets 2009; 20: 229–234.

6. Miller JL. Glycoprotein analysis for the diagnostic evaluation of platelet disorders. Semin Thromb Hemost 2009; 35: 224–232.

7. Giannini S, Mezzasoma AM, Guglielmini G et al. A new case of acquired Glanzmann’s thrombasthenia: diagnostic value of flow cytometry. Cytometry B Clin Cytom 2008; 74: 194–199.

8. Nurden P, Jandrot-Perrus M, Combrié R et al. Severe deficiency of glycoprotein VI in a patient with gray platelet syndrome. Blood 2004; 104: 107–114.

9. Nishibori M, Cham B, McNicol A et al. The protein CD63 is in platelet dense granules, is deficient in a patient with Hermansky-Pudlak syndrome, and appears identical to granulophysin. J Clin Invest 1993; 91: 1775–1782.

10. Lederbogen F, Baranyai R, Gilles M et al. Effect of mental and physical stress on platelet activation markers in depressed patients and healthy subjects: a pilot study. Psychiatry Res 2004; 127: 55–64.

11. Barnard MR, Linden MD, Frelinger AL 3rd et al. Effects of platelet binding on whole blood flow cytometry assays of monocyte and neutrophil procoagulant activity. J Thromb Haemost 2005; 3: 2563–2570.

12. Robert S, Poncelet P, Lacroix R et al. Standardization of platelet-derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies? J Thromb Haemost 2009; 7: 190–197.

13. Nishioka T, Yamane T, Takubo T et al. Detection of various platelet-associated immunoglobulins by flow cytometry in idiopathic thrombocytopenic purpura. Cytometry B Clin Cytom 2005; 68: 37–42.

14. Hézard N, Simon G, Macé C et al. Is flow cytometry accurate enough to screen platelet autoantibodies? Transfusion 2008; 48: 513–518.

15. Dijkstra-Tiekstra MJ, van der Meer PF, Pietersz RN et al. Multicenter evaluation of two flow cytometric methods for counting low levels of white blood cells. Transfusion 2004; 44: 1319–1324.

16. Dijkstra-Tiekstra MJ, Pietersz RN, Huijgens PC. Correlation between the extent of platelet activation in platelet concentrates and in vitro and in vivo parameters. Vox Sang 2004; 87: 257–263.

17. Monteagudo M, Amengual MJ, Muñoz Let al. Reticulated platelets as a screening test to identify thrombocytopenia aetiology. QJM 2008; 101: 549–555.

18. Saigo K, Sakota Y, Masuda Y et al. Automatic detection of immature platelets for decision making regarding platelet transfusion indications for pediatric patients. Transfus Apher Sci 2008; 38: 127–132.

19. Kunz D, Höffkes H, Kunz WS et al. Standardized flow cytometric method for the accurate determination of platelet counts in patients with severe thrombocytopenia. Cytometry 2000; 42: 284–289.

Labels
Diabetology Endocrinology Internal medicine

Article was published in

Internal Medicine

Issue Supplementum 1

2010 Issue Supplementum 1

Most read in this issue
Topics Journals
Login
Forgotten password

Enter the email address that you registered with. We will send you instructions on how to set a new password.

Login

Don‘t have an account?  Create new account

#ADS_BOTTOM_SCRIPTS#