Next-generation sequencing
Authors:
Eva Froňková
Authors place of work:
Laboratoř molekulární genetiky CLIP, Klinika dětské hematologie a onkologie 2. LF UK a FN Motol, Praha
Published in the journal:
Čes.-slov. Patol., 49, 2013, No. 3, p. 129-132
Category:
Přehledový článek
Summary
Next generation or massive parallel sequencing (MPS) is a rapidly advancing method in molecular genetics that will bring significant changes in the personalized medicine field. In this review we briefly describe major types of MPS, including whole-genome, -exome, -transcriptome and amplicon sequencing. We also present an overview of the advantages, drawbacks and possible applications of sequencing technologies available in the Czech Republic.
Keywords:
massive parallel sequencing – next generation sequencing – whole-genome – exome – transcriptome – amplicon sequencing
Zdroje
1. Meyerson M, Gabriel S, Getz G. Advances in understanding cancer genomes through second-generation sequencing. Nat Rev Genet 2011; 11(10): 685–696.
2. Welch JS, Link DC. Genomics of AML: clinical applications of next-generation sequencing. Hematology Am Soc Hematol Educ Program 2011; 30–35.
3. Teer JK, Mullikin JC. Exome sequencing: the sweet spot before whole genomes. Hum Mol Genet 2010; 19(R2): R145–151.
4. Gartner J, Davis S, Wei X, et al. Comparative exome sequencing of metastatic lesions provides insights into the mutational progression of melanoma. BMC Genomics 2012; 13: 505.
5. Malone JH, Oliver B: Microarrays, deep sequencing and the true measure of the transcriptome. BMC Biol 2011; 9: 34.
6. Logan AC, Gao H, Wang C, Sahaf B, et al. High-throughput VDJ sequencing for quantification of minimal residual disease in chronic lymphocytic leukemia and immune reconstitution assessment. Proc Natl Acad Sci USA 2011; 108(52): 21194– 21199.
7. Lister R, Pelizzola M, Dowen RH, et al. Human DNA methylomes at base resolution show widespread epigenomic differences. Nature 2009; 462(7271): 315–322.
8. Glenn TC. Field guide to next-generation DNA sequencers. Mol Ecol Resour 2011; 11: 759–769.
9. Loman NJ, Misra RV, Dallman TJ, et al. Performance comparison of benchtop high-throughput sequencing platforms. Nat Biotechnol 2012; 30(5): 434–439.
10. Liu L, Li Y, Li S, et al. Comparison of next-generation sequencing systems. J Biomed Biotechnol 2012; 251364.
11. Quail MA, Smith M, Coupland P, et al. A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genomics 2012; 13: 341.
Štítky
Patologie Soudní lékařství ToxikologieČlánek vyšel v časopise
Česko-slovenská patologie
![Číslo 3](https://www.prolekare.cz/media/cache/resolve/journal_issue/media/image_pdf/07fa63c7d993e3e6ce488435843185c1.jpeg)
2013 Číslo 3
Nejčtenější v tomto čísle
- Polymerázová řetězová reakce: princip metody a využití v molekulární patologii
- Sekvenování – klasická metodika
- Sekvenování nové generace
- Rád se dívám na svět i jinak než mikroskopem