The Use of Multiplex ImmunoCAP ISAC System for the Assesment of Sensibilization to Molecular Components Related to the Severity of Atopic Dermatitis
Authors:
R. Vaňková 1; J. Čelakovská 2; J. Bukač 3; I. Krčmová 1; J. Krejsek 1; C. Andrýs 1
Authors‘ workplace:
Ústav klinické imunologie a alergologie, Univerzita Karlova, Lékařská fakulta v Hradci Králové, Fakultní nemocnice Hradec Králové, přednosta prof. RNDr. Jan Krejsek, CSc.
1; Klinika nemocí kožních a pohlavních, Univerzita Karlova, Lékařská fakulta v Hradci Králové, Fakultní nemocnice Hradec Králové, přednosta doc. MUDr. Miloslav Salavec, CSc.
2; Ústav lékařské biofyziky, Univerzita Karlova, Lékařská fakulta v Hradci Králové, přednosta prof. Ing. Jan Kremláček, Ph. D.
3
Published in:
Čes-slov Derm, 95, 2020, No. 5, p. 166-179
Category:
Pharmacologyand Therapy, Clinical Trials
Overview
The aim of the study was to evaluate the benefit of testing specific IgE antibodies against molecularly defined allergens in patients with atopic dermatitis (AD) using the multiplex method ISAC and to assess whether the sensitization profiles differ in mild, moderate and severe forms of AD. A total of 81 patients (41 men and 40 women) were included in the study, who underwent a complete dermatological and allergological examination, including the determination of the sensitization profile to molecular components by the ImmunoCAP ISAC method. The results of the examination were statistically processed. A significantly higher number of positive reactions with high levels of specific IgE (≥ 15 ISU – E) was demonstrated in patients with moderate to severe AD. For most molecular components, increasing relative frequencies of positive reactions from mild to severe have been reported. In patients with severe form, sensitization to the molecular components of house dust mites, animals (cats, dogs, horses), egg white, shrimp and fungi (Aspergillus, Alternaria) was significantly more common. In this regard, we recommend focusing examinations, treatment, and regimen measures.
Keywords:
atopic dermatitis – component-resolved diagnostics – ISAC test – SCORAD index
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Dermatology & STDs Paediatric dermatology & STDsArticle was published in
Czech-Slovak Dermatology
2020 Issue 5
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