Supernatanty Lactobacillus rhamnosus GG a Lactobacillus crispatus SJ-3C-US snižují expresi genů TSGA10, AURKC, OIP5 a AKAP4 v HeLa buňkách

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Authors: Z. Nouri ¹; N. Neyazi ¹; MH. Modarressi ²; F. Karami ³; A. Abedin-Do ⁴; Z. Taherian-Esfahani ⁴; S. Ghafouri-Fard ⁴; E. Motevaseli ⁵
Authors‘ workplace: Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran ¹; Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran ²; Department of Medical Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran ³; Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran ⁴; Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran ⁵
Published in: Klin Onkol 2018; 31(6): 429-433
Category: Original Articles
doi: https://doi.org/10.14735/amko2018429

Overview

Východiska:

Nádorové antigeny testis (CTA) jsou považovány za nádorové biomarkery z důvodu jejich vysoce specifické exprese u lidských malignit a jelikož se téměř nevyskytují v normálních somatických tkáních. Díky své specifické expresi umožňují v posledních letech lépe stanovit včasnou diagnózu, prognózu pacientů a léčbu rakoviny. Lactobacily jsou skupina probiotik s protinádorovými, imunomodulačními a dalšími prospěšnými vlastnostmi. Bylo prokázáno, že tyto bakterie mění expresi několika genů souvisejících s nádory.

Cíl:

Po synchronizaci buněk HeLa pomocí MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-difenyltetrazoliumbromid] jsme pomocí kvantitativní polymerázové řetězové reakci v reálném čase zkoumali vliv supernatantu Lactobacillus rhamnosus GG (LRS) a supernatantu Lactobacillus crispatus SJ-3C-US (LCS) na expresi čtyř CTA (TSGA10, AURKC, OIP5 a AKAP4).

Výsledky:

LRS a LCS inhibovaly růst buněk HeLa po 24 hod, což bylo prokázáno pomocí MTT testu. Exprese všech CTA byly po léčbě oběma supernatanty nižší.

Závěr:

Tato studie prokázala úlohu laktobacilů při snížení exprese genů CTA. Taková změna exprese může být zapojena do protinádorových účinků těchto laktobacilů. Základní mechanismy těchto pozorování nejsou jasné, ale v tomto procesu se mohou účastnit epigenetické modulační mechanismy. K posouzení funkčních rolí laktobacilů v modulaci jiných genů souvisejících s nádory je třeba dalších studií.

Klíčová slova:

probiotika – nádorové antigeny testis – biomarker – HeLa buněčná linie

Tato studie byla podpořena Teheránskou univerzitou lékařských věd. Autoři děkují členům Genetické a biotechnologické laboratoře za pomoc při výzkumu.

Autoři deklarují, že v souvislosti s předmětem studie nemají žádné komerční zájmy.

Redakční rada potvrzuje, že rukopis práce splnil ICMJE kritéria pro publikace zasílané do bi omedicínských časopisů.

Obdrženo: 19. 8. 2018

Přijato: 30. 9. 2018

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