VYUŽITIE MULTIPLEXNEJ RT PCR V DIAGNOSTIKE MIKROBIONÁLNYCH PATOGÉNOV

Czech version

Authors: Marianna Trebuňová ^1,2; Ivan Vanát ¹; Zuzana Vaczy ¹; Ján Rosocha ¹; Ladislav Bobák ¹; Jozef Živčák ²
Authors‘ workplace: Združená tkanivová banka UNLP Košice, Košice, Slovensko ¹; Katedra biomedicínskeho inžinierstva a merania SjF TU, Košice, Slovensko ²
Published in: Lékař a technika - Clinician and Technology No. 3, 2016, 46, 74-77
Category: Original research

Overview

The aim of this paper is to show the potential of using a diagnostic kit Anyplex^TM STI-7 (Seegene, South Korea), which uses technology to multiplex real time PCR. We can detect the seven of the most common sexually transmitted pathogens simultaneously in one PCR reaction with use Anyplex^TM STI-7. These are the six microbial pathogens (Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum and Ureaplasma parvum) and the protozoan (Trichomonas vaginalis). According to the European Guidelines for management of non-gonococcal urethritis of 2016 are infections caused by Chlamydia trachomatis and Mycoplasma genitalium most frequently reported sexually transmitted diseases. Chlamydia trachomatis and Mycoplasma genitalium are the most commonly reported sexually transmitted diseases. Using the multiplex real time PCR Anyplex^TM STI-7, we found out that in our group of infertile men was Ureaplasma parvum most frequently detected pathogen.

Keywords:
Multiplex real time PCR, Anyplex^TM STI-7, Chlamydia, Mycoplasma

Sources

[1] Maruška, M.; Darja, K.; Jovan, M.; Mojca, M.: Clinical role of Ureaplasma parvum and Ureaplasma urealyticum presence in female lower urogenital tract: is there a place for routine screening and treatment?. Zdrav. Vestn. 2014, 83: 629–637.

[2] WHO Sexually transmitted infections. World Health Organization 2013; Available November 15, 2013 from: http://www.who.int/mediacentre/factsheets/fs110/en/.

[3] World Health Organisation. Sixty-ninth World Health Assembly, document WHA69/2016/REC/1, GLOBAL HEALTH SECTOR STRATEGY ON SEXUALLY TRANSMITTED INFECTIONS 2016–2021, TOWARDS ENDING STIs, June 2016, 64 pp.

[4] Horner, P.J.; Blee, K.; Falk, L.; van der Meijden, W.; Moi, H.: European Guideline on the management of non-gonococcal urethritis. 2016, 26 pp.

[5] Holmes, K.K.; Stamm, W.E.; Sobel, J.D.: Lower genital tract infection syndromes in women. In: Holmes KK, Sparling PF, Stamm WE, Piot P, Wasserheit JN, Corey L, et al, eds. Sexually transmitted diseases. 4th ed. New York: McGraw-Hill; 2008. p. 987–1016.

[6] Povlsen, K.; Bjornelius, E.; Lidbrink, P.; Lind, I.: Relationship of Ureaplasma urealyticum biovar 2 to nongonococcal urethritis. Eur J Clin Microbiol Infect Dis 2002; 21: 97–101.

[7] Deguchi, T.; Yoshida, T.; Miyazawa, T.; Yasuda, M.; Tamaki, M.; Ishiko, H., et al.: Association of Ureaplasma urealyticum (biovar 2) with nongonococcal urethritis. Sex Transm Dis 2004; 31: 192–5.

[8] Couldwell, D.L.; Gidding, H.F.; Freedman, E.V.; McKechnie, M.L.; Biggs, K.; Sintchenko, V., et al.: Ureaplasma urealyticum is significantly associated with non-gonococcal urethritis in heterosexual Sydney men. Int J STD AIDS 2010; 21: 337–41.

[9] Ondondo, R.O.; Whittington, W.L.; Astete, S.G.; Totten, P.A.: Differential association of ureaplasma species with non-gonococcal urethritis in heterosexual men. Sex Transm Infect 2010; 86: 271–5.

[10] Maeda, S.; Deguchi, T.; Ishiko, H.; Matsumoto, T.; Naito, S.; Kumon, H., et al.: Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum (biovar 1) and Ureaplasma urealyticum (biovar 2) in patients with non-gonococcal urethritis using polymerase chain reaction-microtiter plate hybridization. Int J Urol 2004; 11: 750–4.

[11] Chun, J. Y.: Multiplex molecular diagnostics: shifting the paradigm. In: Medical laboratory observed. 2012; Seegene bulletin, Vol.1.

[12] Choe, H. S.; Lee, D. S. et al.: Performance of AnyplexTM II multiplex RT PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods. IJID. 17(12): 1134-1140. 2013. ISSN 1201-9712.

[13] DPO ^TM Technology. Novel Oligo platformo f super multiplex PCR. URL: http://seegene.com/neo/en/introduction/core_dpo.php

[14] TOCE ^TM Technology. The New Paradigm for High multiplex Real/time PCR. URL: http://seegene.com/neo/en/introduction/core_toce.php

[15] Kweon, O.J.; Lim, Y.K.; Oh, S.M.; Kim, T.H.; Choe, H.S.; Lee, S.J.; Cho, Y.H.; Lee, M.K.: Prevalence and Antimicrobial Susceptibility of Mycoplasma hominis, Ureaplasma urealyticum and Ureaplasma parvum in Individuals With or Without Symptoms of Genitourinary Infections. Lab Med Online. 2016 Apr;6(2):79-87. URL: https://doi.org/10.3343/lmo.2016.6.2.79.

Labels

Biomedicine

ANTIBACTERIAL ACTIVITY OF TITANIUM DIOXIDE AND AG-INCORPORATED DLC THIN FILMS

RESPIRATORY ARRHYTHMIA AS AN ENCOURAGEMENT OF INAPPROPRIATE ICD THERAPY

Article was published in

The Clinician and Technology Journal

2016 Issue 3