Vliv ultrazvuku na účinnost fotodynamické terapie – in vitro studie
Authors:
Klára Pížová 1,2; Kateřina Tománková 1; Kateřina Langová 1; Adéla Hanáková 1,2; Hana Lenobelová 1; Hana Zapletalová 1; Jakub Malohlava 1,2; Svatopluk Binder 1; Robert Bajgar 1,2; Jaromír Vachutka 1; Ladislav Doležal 1; Hana Kolářová 1
Authors‘ workplace:
Ústav lékařské biofyziky, Lékařská fakulta, Univerzita Palackého, Olomouc, ČR
1; Ústav molekulární a translační medicíny, Lékařská fakulta, Univerzita Palackého, Olomouc, ČR
2
Published in:
Lékař a technika - Clinician and Technology No. 4, 2012, 42, 18-22
Overview
Photodynamic therapy (PDT) belongs in perspective modalities of cancer treatment. It is based on the tumour-selective accumulation of a photosensitizer followed by irradiation with light of a specific wavelength. PDT is widely developed nowadays due to its high specificity and selectivity along with absence of the unadvisable side-effects. Sonodynamic therapy (SDT) exploits ultrasound to induce cytotoxic effect of sensitizer. In our study we tested the possibility of combination of this therapies and icrease of efficiency. Our results suggest that irradiation in combination with application of therapeutic ultrasound increases production of reactive oxygen species and reduces viability of tumour MCF7 cells, compared to irradiation of ZnTPPS4 only, especially in the case of higher therapeutic doses. In the future, the combination of PDT and SDT can bring a new treatment modality for malignant and also nonmalignant diseases.
Keywords:
photosensitizer, photodynamic therapy, sonodynamic therapy, porphyrine derivative
Sources
[1] Pushpan, S.K., Venkatraman, S., Anand, V.G., Sankar, J., Parmeswaran, D., Ganesan, S., Chandrashekar, T.K.: Porphyrins in photodynamic therapy - a search for ideal photosensitizers. Curr Med Chem Anticancer Agents, 2, 2002, pp. 187-207.
[2] Calzavara-Pinton, P.G., Venturini, M., Sala, R.: Photodynamic therapy: update 2006. Part 1: Photochemistry and photobiology. J Eur Acad Dermatol Venereol, 21, 2007, pp. 293-302.
[3] O'Connor, A.E., Gallagher, W.M., Byrne, A.T.: Porphyrin and nonporphyrin photosensitizers in oncology: preclinical and clinical advances in photodynamic therapy. Photochem Photobiol, 85, 2009, pp. 1053-1074.
[4] Kudinova, N.V., Berezov, T.T.: Photodynamic Therapy of Cancer: Search For Ideal Photosensitizer. Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 4, 2010, pp. 95–103.
[5] Agostinis, P., Berg, K., Cengel, K.A., Foster, T.H., Girotti, A.W., Gollnick, S.O., Hahn, S.M., Hamblin, M.R., Juzeniene, A., Kessel, D., Korbelik, M., Moan, J., Mroz, P., Nowis, D., Piette, J., Wilson, B.C., Golab, J.: Photodynamic therapy of cancer: an update. CA Cancer J Clin, 61, 2011, pp. 250-281.
[6] Suslick, K.S.: Sonochemistry. Science, 247, 1990, pp. 1439-1445.
[7] Worthington, A.E., Thompson, J., Rauth, A.M., Hunt, J.W.: Mechanism of ultrasound enhanced porphyrin cytotoxicity. Part I: A search for free radical effects. Ultrasound Med Biol, 23, 1997, pp. 1095-1105.
[8] Miyoshi, N., Takeshita, T., Misik, V., Riesz, P.: Monomerization of photosensitizers by ultrasound irradiation in surfactant micellar solutions. Ultrason Sonochem, 8, 2001, pp. 367-371.
[9] Rosenthal, I., Sostaric, J.Z., Riesz, P.: Sonodynamic therapy - a review of the synergistic effects of drugs and ultrasound. Ultrason Sonochem, 11, 2004, pp. 349-363.
[10] Hiraoka, W., Honda, H., Feril, L.B. Jr., Kudo, N., Kondo, T.: Comparison between sonodynamic effect and photodynamic effect with photosensitizers on free radical formation and cell killing. Ultrason Sonochem, 13, 2006, pp. 535-542.
[11] Jin, Z.H., Miyoshi, N., Ishiguro, K., Umemura, S., Kawabata, K., Yumita, N., Sakata, I., Takaoka, K., Udagawa, T., Nakajima, S., Tajiri, H., Ueda, K., Fukuda, M., Kumakiri, M.: Combination effect of photodynamic and sonodynamic therapy on experimental skin squamous cell carcinoma in C3H/HeN mice. J Dermatol, 27, 2000, pp. 294-306.
[12] Yumita, N., Nishigaki, R., Umemura, S.: Sonodynamically induced antitumor effect of Photofrin II on colon 26 carcinoma. J Cancer Res Clin Oncol, 126, 2000, pp. 601-606.
[13] Yumita, N., Umemura, S.: Sonodynamic therapy with photofrin II on AH130 solid tumor. Pharmacokinetics, tissue distribution and sonodynamic antitumoral efficacy of photofrin II. Cancer Chemother Pharmacol, 51, 2003, pp. 174-178.
[14] Milowska, K., Gabryelak, T.: Synergistic effect of ultrasound and phthalocyanines on nucleated erythrocytes in vitro. Ultrasound Med Biol, 31, 2005, pp. 1707-1712.
[15] Kolarova, H., Bajgar, R., Tomankova, K., Krestyn, E., Dolezal, L., Halek, J.: In vitro study of reactive oxygen species production during photodynamic therapy in ultrasound-pretreated cancer cells. Physiol Res, 56Suppl 1), 2007, pp. S27-32.
[16] Kolarova, H., Tomankova, K., Bajgar, R., Kolar, P., Kubinek, R.: Photodynamic and sonodynamic treatment by phthalocyanine on cancer cell lines. Ultrasound Med Biol, 35, 2009, pp. 1397-1404.
[17] Wang, J., Guo, Y., Gao, J., Jin, X., Wang, Z., Wang, B., Li, K., Li, Y.: Detection and comparison of reactive oxygen species (ROS) generated by chlorophyllin metal (Fe, Mg and Cu) complexes under ultrasonic and visible-light irradiation. Ultrason Sonochem, 18, 2011, pp. 1028-1034.
[18] El-Sikhry, H.E., Miller, G.G., Madiyalakan, M.R., Seubert, J.M.: Sonodynamic and photodynamic mechanisms of action of the novel hypocrellin sonosensitizer, SL017: mitochondrial cell death is attenuated by 11, 12-epoxyeicosatrienoic acid. Invest New Drugs, 29, 2011, pp. 1328-1336.
Labels
BiomedicineArticle was published in
The Clinician and Technology Journal
2012 Issue 4
Most read in this issue
- Difficulties of thermographic measurements in medicine
- Fantom pro diagnostický ultrazvuk a dopplerovské vyšetření
- Vliv ultrazvuku na účinnost fotodynamické terapie – in vitro studie
- Vliv poruch sondy sonografu na kvalitativní parametry ultrazvukového B-obrazu