Assuring reliability of blood count examinations

Czech version

Authors: M. Matýšková; L. Bourková; J. Hoblová
Authors‘ workplace: Oddělení klinické hematologie FN Brno, pracoviště Bohunice, primář prof. MUDr. Miroslav Penka, CSc.
Published in: Vnitř Lék 2005; 91(7 a 8): 894-899
Category: 128th Internal Medicine Day - 21rd Vanysek's Day Brno 2005

Overview

Blood count examination belongs to the basic laboratory examinations. Therefore it is necessary to dedicate sufficient care both from the side of the clinician and of the laboratory staff. Like by other laboratory examinations, including patient's clinical information on the request form, and on accurate interpretation both from the laboratory and clinician. The clinician should have at least a basic knowledge about possibilities of the laboratory; about testimonial value of individual assessments and about possible interferences and cross reactions. The laboratory has to have a well documented control system which includes not only passages about laboratory equipment care but also the examination procedures themselves (internal and external quality control). From the above mentioned we can conclude that the pre-examination, examination and post-examination procedures are interconnected and so close cooperation between clinicians and the lab is necessary get correct results.

Key words:
quality control - pre-examination procedures - analytic procedure - post-examination procedures - blood count evaluation

Sources

1. Cott van EM, Lewandrowski KB, Patel S et al. Comparison of glass K3EDTA versus plastic K2EDTA Blood Drawing tubes for complete blood counts, reticulocyte count, and white blood cell differentials. Lab Hematol 2003; 9: 10-14.

2. ČSN EN ISO 15189. 2004 Zdravotnické laboratoře - Zvláštní požadavky na jakost a způsobilost. ČSNI Praha 2004.

3. Gullati GL, Piao YF, Song AS et al. Interference by cryoproteins in the blood with automated CBCs. Lab Med 1995; 26: 138-141.

4. Reference and Selected Procedures for the Quantitative Determination of Hemoglobin in Blood. 2nd ed. NCCLS document. Wayne 1994.

5. Hoffmann JJML. How useful are hematology analyse flags? Clin Chem Lab Med 2004; 42: 357-358.

6. Meer van der W, Scott CS, de Keijzer MH. Automated flagging influences the inconsistency and bias of band and atypical lymphocyte morphological differentials. Clin Chem Lab Med 2004; 42: 371-377.

7. Mellors I, McArdle P, Bell D. Pseudoleucocytosis due to incomplete erytrocyte lysis. Clin Lab Hamat 1995; 17: 347-350.

8. Calibration and control of basic blood cell counters. WHO Document LAB/97.2 (1997).

9. Lewis SM. Standardization and harmonization of the blood count: The role of International Committee for Standardization in Haematology (ICSH). Eur J Haematol 1990, 45(Suppl 53): 9-13.

10. Commission decision on Common Technical Specifications for in vitro-diagnostic medical devices. Official Journal of the European Communities 2002; L 131: 17-30.

11. ČSN EN ISO 375: 2001 Informace předávané výrobcem s diagnostickými činidly in vitro pro odborné použití. ČSNI Praha 2001.

12. ISO/CD TR 18112: 2004 ISO - In vitro diagnostic medical devices - Summary of regulatory requirements for information supplied by the manufacturer. ISO 2004.

13. Howard CA. An atypical spike in a platelet histogram caused by a type I cryoglobulinaemia. Med 1994; 25: 770-774.

14. Bessman JD, Gilmer PR, Gardner FH. Improved classification o anaemias by MCV and RDW. Am J Clin Pathol 1983; 80: 332-335.

15. Quality assurance in haematology. WHO Document LAB/98.4 (1998).

16. Bourková L, Matýšková M, Kratochvíla J. Doporučení ČHS ČLS JEP: Kontrola kvality měření krevních obrazů na hematologických analyzátorech. 2004. www.hematology.cz

17. ISO 13528: 2003 Statistical methods for use in proficiency testing by inter-laboratory comparisons. ISO 2003.