Real-time PCR detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae DNA in clinical specimens
Authors:
Z. Vacková 1,2; D. Lžičařová 1,3; N. K. Stock 1,4,5; J. Kozáková 1
Authors‘ workplace:
Centrum epidemiologie a mikrobiologie, Státní zdravotní ústav, Praha
1; 3. lékařská fakulta Univerzity Karlovy v Praze
2; Ústav lékařské mikrobiologie 2. LF UK a FN Motol
3; European Program for Public Health Microbiology (EUPHEM), ECDC, Stockholm, Sweden
4; Institute of Hygiene and Microbiology, Julius-Maximilians-University, Würzburg, Germany
5
Published in:
Epidemiol. Mikrobiol. Imunol. 64, 2015, č. 4, s. 222-230
Category:
Original Papers
Overview
The study aim was to implement a molecular real-time polymerase chain reaction (PCR) assay recommended by the CDC (Centers for Disease Control and Prevention) for the detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical (culture negative) specimens from patients with suspected invasive bacterial disease. Clinical specimens are referred to the National Reference Laboratory (NRL) for Meningococcal Infections, Unit for Airborne Bacterial Infections, Centre for Epidemiology and Microbiology, National Institute of Public Health from various regions of the Czech Republic. Clinical specimens are, in particular, cerebrospinal fluid, anti-coagulated blood or serum and, exceptionally, post-mortem specimens. The NRL has implemented molecular diagnosis of these bacterial pathogens involved in meningitis and sepsis from clinical specimens since 1999. The first diagnostic method was semi-nested PCR followed by electrophoretic analysis. In 2014, a molecular qualitative real-time PCR assay was implemented.
Keywords:
rt-PCR detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae – rt-PCR detection of the agents of bacterial meningitis and sepsis
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Hygiene and epidemiology Medical virology Clinical microbiologyArticle was published in
Epidemiology, Microbiology, Immunology
2015 Issue 4
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