Immunohistochemical Detection and Quantification of Minimal ResidualDisease with the Use of DBA.44 Antibody in Trephine Bone Marrow Biopsiesof Patients with Hairy Cell Leukemia
Authors:
K. Dědič 1; P. Žák 2
Authors‘ workplace:
Fingerlandův ústav patologie, Lékařská fakulta v Hradci Králové, Univerzita Karlova, Praha2Oddělení klinické hematologie Fakultní nemocnice, Hradec Králové
1
Published in:
Čes.-slov. Patol., , 2003, No. 4, p. 175-177
Category:
Overview
Hairy cell leukemia patients treated with 2-chlorodeoxyadenosine or 2-deoxycoformicin achievea very high number of therapeutic responses. After complete remission, i.e. minimal residualdisease, we cannot demonstrate the disease activity, splenomegaly, or lymphadenopathy; moreover,there comes to normalization of hemoglobin concentration, leukocyte count, and plateletcount. No leukemic cells in peripheral blood or bone marrow smears can be seen with the use ofstaining techniques. They can be demonstrated immunohistochemically in decalcified trephinebone marrow biopsies with the use of DBA.44 antibody together with their morphologic features.For quantification of leukemic cells we use LUCIA-M image analysis.
Key words:
hairy cell leukemia – immunohistochemistry – minimal residual disease – trephinebone marrow biopsy – DBA.44
Labels
Anatomical pathology Forensic medical examiner ToxicologyArticle was published in
Czecho-Slovak Pathology
2003 Issue 4
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