#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Apoptosis and Expression of bcl-2 Protein in Invasive DuctalAdenocarcinoma of the Pancreas


Authors: J. Brázdil 1;  Z. Lukáš 1;  M. Hermanová 1;  M. Pazourková 2;  M. Růžička 3;  B. Habanec 1;  L. Křen 1;  Petr Dítě
Authors‘ workplace: Patologicko-anatomický ústav FN, Brno 2 Interní gastroenterologická klinika FN, Brno3 Chirurgické oddělení nemocnice Delta, Brno 1
Published in: Čes.-slov. Patol., , 2003, No. 4, p. 168-173
Category:

Overview

Apoptosis plays a central role in the development and/or progression of cancer. There are severalmethods for detection of apoptotic cells in tissue sections including light and electron microscopy,in situ nick end-labeling (ISEL), TdT-mediated dUTP nick-end labeling (TUNEL) and immunohistochemicaldetection of proteins associated with apoptosis. Apoptosis was assessed by the monoclonalantibody M30 CytoDEATH (M30), which is specific for neo-epitope in cytokeratin 18 thatbecomes available at an early caspase cleavage during apoptosis. Expression of bcl-2 protein wasevaluated, because bcl-2 protein plays an important role in the regulation of apoptosis. Twentysixinvasive ductal adenocarcinomas of the pancreas were studied immunohistochemically withantibodies M30 and bcl-2. The mean apoptotic index (AI, the percentage of apoptotic cells of thetotal tumor cells number) was 2.75%. High AI (>10%) was observed in 4 cases of the 26 pancreaticcarcinomas (15%). Protein bcl-2 was expressed in 3 cases (11,5 %). The AI did not correlate withthe expression of protein bcl-2. In conclusion, the detection of neo-epitope in cytokeratin 18 bymonoclonal antibody M30 can be used for quantification of apoptotic cells with immunohistochemicaltechniques in tissue sections. It is a new approach to evaluate apoptosis in pancreaticcarcinomas. The low positivity of bcl-2 expression in pancreatic adenocarcinomas suggests thatbcl-2 protein does not play a central role in pancreatic tumorigenesis and cancer progression.

Key words:
apoptosis – bcl-2 – pancreatic carcinoma – monoclonal antibody M30

Full text is not available online.
If interested in a scan of this journal, contact NTO ČLS JEP.

Labels
Anatomical pathology Forensic medical examiner Toxicology
Topics Journals
Login
Forgotten password

Enter the email address that you registered with. We will send you instructions on how to set a new password.

Login

Don‘t have an account?  Create new account

#ADS_BOTTOM_SCRIPTS#