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Possibilities of Age Assessment by Peptide Mapping of Non-collagenous Proteins of Human Dentin


Authors: Sajdok J.~;  A. Kotrbová-Kozak 1;  A. Pilin 2
Authors‘ workplace: lÚstav biochemie a mikrobiologie, Fakulta potravinářské a biochemické technologie, VŠCHT, Praha 2Ústav soudního lékařství a toxikologie 1. LF UK a VFN, Praha
Published in: Čes.-slov. Patol., , 2001, No. 1, p. 5-8
Category:

Overview

The submitted paper deals with possibilities of assessment of human age based on findings of stereospecific breakdown of proteins containing D-formy of aspartic acid.The stereospecificity of enzymatic breakdown assumes that after enzymatic hydrolysis peptide breakdown producta with different molecular weights - the so-called peptide map - will be formed, depending how many D-aspartyl residues the protein contains. The suthors proved in the submitted preliminary study in subjects of different age the formation of breakdown products of different size in non-collagenous proteins of human dentin which was hydrolyzed by protease VS from Staphylococcus aureus.

Key words:
assessment of human age, d-aspartic acid, non•collagenous dentin proteins, breakdown by protease from Staphylococus aureus, peptide map

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Anatomical pathology Forensic medical examiner Toxicology
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