Development of PCR Methods and Their Applications in Oncological Research and Practice

Czech version

Authors: R. Hrstka; T. Kolářová; E. Michalová; B. Vojtěšek
Authors‘ workplace: Regionální centrum aplikované molekulární onkologie, Masarykův onkologický ústav, Brno
Published in: Klin Onkol 2014; 27(Supplementum): 69-74

Overview

Since its discovery, PCR has become a conventional method of molecular biology research laboratories and an indispensable tool in diagnostic medicine. Multiple variants of the PCR technique were developed, which enable the analysis of different biological materials at different amounts and reaction conditions. This article briefly summarizes the PCR approaches and points out their applications in oncological research and practice.

Key words:
polymerase chain reaction (PCR) – real‑ time PCR – digital PCR – clinical oncology

This work was supported by the European Regional Development Fund and the State Budget of the Czech Republic (RECAMO, CZ.1.05/2.1.00/03.0101), BBMRI_CZ (LM2010004), GACR 13-00956S and by MH CZ – DRO (MMCI, 00209805).

The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study.

The Editorial Board declares that the manuscript met the ICMJE “uniform requirements” for biomedical papers.

Submitted:
10. 2. 2014

Accepted:
1. 4. 2014

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