Analýza imunohistochemického a genetického vzorce exprese kisspeptinu v endometriálních polypech

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Autoři: Şeyma İlayda Paltacı ¹ ; Özlem Kayacık Günday ¹ ; Müjgan Özdemir Erdoğan ² ; Fatma Fırat ³ ; Gülsüm Şeyma Yalçın ⁴ ; Nermin Akçalı ²
Působiště autorů: Department of Obstetrics and Gynecology, Faculty of Medicine, Afyonkarahisar University of Health Sciences, Afyonkarahisar, Turkey ¹; Department of Genetics, Faculty of Medicine, Afyonkarahisar University of Health Sciences, Afyonkarahisar, Turkey ²; Department of Histology and Embryology, Faculty of Medicine, Afyonkarahisar University of Health Sciences, Afyonkarahisar, Turkey ³; Department of Pathology, Faculty of Medicine, Afyonkarahisar University of Health Sciences, Afyonkarahisar, Turkey ⁴
Vyšlo v časopise: Ceska Gynekol 2024; 89(4): 269-277
Kategorie: Původní práce
doi: https://doi.org/10.48095/cccg2024269

Souhrn

Cíl: Endometriální polypy (EP) představují patologii, která je v klinické praxi běžná. Ačkoli jejich přesná etiologie není úplně známá, existují důkazy o tom, že jsou citlivé vůči hormonální stimulaci. Naším cílem bylo prozkoumat vztah mezi kisspeptinem (KP) a EP pomocí srovnání míry genetické exprese (tkáň–krev) a imunohistochemické (IHC) exprese KP v lézích EP u pacientek s normálním endometriálním nálezem. Materiál a metody: Byla provedena retrospektivní případová studie u 50 pacientek s EP (n = 25) a normálním endometriálním nálezem (n = 25) z biopsie a/nebo materiálu z excize. Krev a vzorky z biopsie od všech pacientek byly uchovávány při –80 °C. Exprese genů KP byla stanovena v parafinových blocích a vzorcích periferní žilní krve získaných z bioptických vzorků a analýza skóre IHC-H byla provedena na parafinových blocích. EP a příslušné kontroly byly srovnány z hlediska KP. Výsledky: Po IHC bylo KP-H skóre v kontrolní skupině vyšší než ve skupině s EP a tento rozdíl byl statisticky významný; H skóre: kontroly: 5 (++; 1–15); polypy: 1 (+; 0–12) (p < 0,05). Ačkoli byla exprese KP jak v tkáni, tak v krvi vyšší v kontrolní skupině oproti skupině s EP, tento rozdíl nebyl statisticky významný (p > 0,05). V krvi ani tkáni nebyla zjištěna významná korelace mezi IHC-H skóre a expresí KP. Podle analýzy ROC cut-off hodnoty exprese KP v tkáni a krvi a plocha pod křivkou (AUC), která predikuje pravděpodobnost vzniku EP, nebyly významné (KP v tkáni: 1,04; AUC: 0,570; p = 0,388; senzitivita 56 %, specificita 60 % / KP v krvi: 1,06; AUC: 0,569; p = 0,401; senzitivita 80 %, specificita 40 %). Závěry: Snížená míra exprese KP v lézích EP může predikovat diagnózu EP a v budoucnu může mít KP při benigních gynekologických patologiích, jako jsou polypy, terapeutický potenciál.

Klíčová slova:

imunohistochemie – endometrium – endometriální polypy – kisspeptin – PCR s reverzní transkripcí (RT-PCR)

Zdroje

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Authorship

ÖKG, SİP: concept and design

ÖKG, MÖE, FF, GŞY, NA: ccquisition, analysis, or interpretation of data

ÖKG, SİP: drafting of the manuscript

ÖKG, MÖE, FF, GŞY, NA: critical review of the manuscript for important intellectual content

ÖKG, MÖE, FF, GŞY, NA: supervision

ORCID authors

Ş. İ. Paltacı 0009-0006-1038-2075

Ö. K. Günday 0000-0002-9249-679X

M. Ö. Erdoğan 0000-0002-3434-8545

F. Fırat 0000-0003-0027-5138

G. Ş. Yalçın 0000-0002-1944-2955