INVAZIVNÍ DUKTÁLNÍ KARCINOMY MLÉČNÉ ŽLÁZY:VYŠETŘENÍ POČTU KOPIÍ GENU CCND1 A POČTUCHROMOZÓMŮ 11 METODOU FLUORESCENČNÍ IN SITUHYBRIDIZACE (FISH) V POROVNÁNÍ S EXPRESÍPROTEINU CYKLIN D1 A RECEPTORU PRO ESTROGEN(ER
) DETEGOVANOU IMUNOHISTOCHEMICKY (IHC)

Invasive Duct Carcinoma of the Breast: Copy Numbers of CCND1 Gene andChromosome 11 Using Fluorescence in situ Hybridization (FISH) in Comparison with the Expression of Cyclin D1and Estrogen Receptor (ER
) Detected by Immunohistochemistry (IHC)

Background.
Overexpression of oncogenic proteins may be caused by gene amplifications. Cyclin D1 participatesin regulation of the cell cycle. Relations between cyclin D1 expression and amplification of CCND1 gene encodingthis protein in invasive duct breast carcinomas (IDC) are not fully elucidated. An increased interest is also focusedon relations to the estrogen receptor (ER
).Methods and Results.We investigated copy numbers of the CCND1 gene, expression of cyclin D1 and expressionof ER
in a group of 60 females and 1 male with IDC. The age range varied from 33 to 89 years (median 57 years).The number of CCND1 gene copies and the number of chromosome 11 was evaluated using FISH, the expressionof cyclin D1 and ER
was investigated by IHC. We detected a strong amplification of CCND1 gene (>10 copies pertumor cell nuclei) in 9 patients, weak amplification (

Key words:
mammary gland, invasive duct carcinoma, CCND1 gene, amplification, cyclin D1, estrogen receptor, FISH.