Changes Levels of Severe Th1, Th2 and Treg Cytokines in Blood Serum of Chronic Infection (incl. H. pylori) in Oropharynx Followed the EPA and DHA Intake and/or Tonsillectomy – a Pilot Study
Authors:
J. Astl 1,2; E. Nártová 4; J. Rotnágl 1; T. Filipovský 1; J. Betka jr. 2; M. Chovanec 2,4; O. Šušoliaková 3; I. Šterzl 3
Authors‘ workplace:
Klinika otorinolaryngologie a maxilofaciální chirurgie 3. LF UK a ÚVN Praha, přednosta prof. MUDr. J. Astl, CSc.
1; Klinika otorinolaryngologie a chirurgie hlavy a krku 1. LF UK a FN Motol, Praha, přednosta prof. MUDr. J. Betka, DrSc.
2; Endokrinologický ústav Praha, ředitelka doc. RNDr. B. Bendlová, CSc.
3; Klinika otorinolaryngologie 3. LF UK a FN Královské Vinohrady, Praha, přednosta MUDr. M. Chovanec, Ph. D.
4
Published in:
Otorinolaryngol Foniatr, 65, 2016, No. 3, pp. 163-167.
Category:
Original Article
Overview
Introduction:
The effect of eikospentan acid (EPA) and dokosahexan acid (DHA) on immune reactions was very intensely studied. The aim of this study was to clarify the impact of chronic Helicobacter pylori infection and EPA and DHA levels on Th1 cytokines (IL-2, IL12-p70, INF-g), Th2 cytokines (IL-4, IL-6) and T reg cytokines (IL-10, TGF-b1) in patient after tonsillectomy.
Materials and methods:
Patients were sorted into three main groups and one control group. Sampling was performed before the start of surgery or before the start of EPA and DHA administration. The second sampling was performed after 15 weeks in all main groups. The daily dosage of 540 mg of EPA and 360 mg of DHA was divides into three doses . The cytokines were analysed from the blood plasma. The concentation of the cytokines was quantified by Quantibody Custom Array. Statistical analysis was performed by using StatGraphics programme – multifactorial analysis ANOVA.
Results:
Statistically significant result was obtained in cytokine IL12-p70 in both measurements. In first sampling (2012) was the significance p=0.04 among the individual groups. In the second sampling the significance was p=0.01 in the phase before and after preparate administration. Dependence on IL-6 was prooved after the perparate admonistration (correlative coeficient 0.48), on IL-4 was dependence little bit higher (correlative coeficient 0.77).
Conclusion:
Cytokine IL12-p70 seems to be the main regulatory cytokine, thus it is possible to think about Th1 and NK cells stimulation.
Keywords:
acid, docosahexanoic acid, interleukins, chronic tonsillitis, cytokines
Sources
1. Achyut, B. R., Ghoshal, U. C., Moorchung, N., Mittal, B.: Transforming growth factor-B1 and matrix metalloproteinase-7 promoter variants induce risk for Helicobacterpylori-associated gastric precancerous lesions. DNA Cell Biol., 28, 2009, 6, s. 295-301.
2. Diehl, S. L, Rincón, M. T:. He two faces of IL-6 on Th1/Th2 differentiation. Mol. Immunol., 39, 2002, 9, s. 531-536.
3. Fujieda, S. L, Suzuji, S., Sunagm H., Yamamoto, H., Seki, M., Sugimoto, H., Saito, H.: Production of interferon-gamma by tonsillar mononuclear cells in IgA nephropathy patients. Acta Otolaryngol., 120, 2000, 5, s. 649-654.
4. Chizzolini, C. L, Chicheportiche, R., Burger, D., Dayer, J. M.: Human Th1 cells preferentially induce interleukin (IL)-1beta while Th2 cells induce IL-1 receptor antagonist production upon cell/cell contact with monocytes. Eur J. Immunol., 27, 1997, 1, s. 171-177.
5. Kong, W., Yen, J. H., Ganea, D.: Docosahexaenoic acid prevents dendritic cell maturation, inhibits antigen-specific Th1/Th17 differentiation and suppresses experimental autoimmune encephalomyelitis. Brain Behav. Immun., 25, 2011, 5, s. 872-882.
6. Kučera, T., Pácová, H., Veselý, D., Astl, J., Martínek, J.: Apoptosis and cell proliferation in chronic tonsillitis and oropharyngeal carcinoma: role of nitroc oxide and cytokines. Biomed. Papers, 148, 2004, 2, s. 225-227.
7. Lukes, P., Pácová, H., Kucera, T., Veselý, D., Martínek, J., Astl, J.: Expression of endothelial and inducible nitric oxide synthase and caspase-3 in tonsillar cancer, chronic tonsillitis and healthy tonsils. Folia Biol. (Praha), 54, 2008, 5, s. 141-145.
8. Razavi, A., Bagheri, N., Azadegan-Dehkordi, F. et al.: Comparative immune response in children and adults with H. pylori infection. J. Immunol. Res., 2015, doi: 10.1155/2015/315957.
9. Schwager, J., Richard, N., Riegger, C., Salem, N. Jr.: ω-3 PUFAs and resveratrol differently modulate acute and chronic inflammatory processes. Biomed Res. Int., 2015; doi: 10.1155/2015/535189
10. Schultze, J., Michalak, S., Lokne, J., Wong, A., Gilleece, M. H., Gribben, J. G., Nadler, L. M.: Human non-germinal center B cell interleukin (IL)-12 production is primarily regulated by T cell signals CD40 ligand, interferon gamma, and IL-10: role of B cells in the maintenance of T cell responses. J. Exp. Med., 189, 1999, 1, s. 1-12.
11. Shaikh, S. R., Editin, M.: Polyunsaturated fatty acids, membrane organization, T cells and antigen presentation. Am. J. Clin. Nutr., 84, 2006, 6, s. 1277-1289.
12. Shaikh, S. R., Editin, M.: Immunosuppressive effects of polyunsaturated fatty acids on antigen presentation by human leukocyte antigen class I molecules. J. Lipid Res., 48, 2007, 1, s. 127-138.
13. Sijben, J. W., Calder, P. C.: Differential immunomodulation with long-chain n-3 PUFA in health and chronic disease. Proc. Nutr. Soc., 66, 2007, 2, s. 237-259.
14. Whelan, J., Goudy, K. M., Shaikh, S. R.: N-3 polyunsaturated fatty acids modulate B cell activity in pre-clinical models: Implications for the immune response to infections. Eur J. Pharmacol., 2015, pii: S0014-2999(15)00465-3. doi: 10.1016/j.ejphar.2015.03.100.
15. Rubin, G., Vebel, P., Briho-Hayek, A. et al.: Validation of a brief symptom questionnaire (REQUES IN PRACTICE) for patients it gastro-oesophageal reflex disease. Aliment Pharmacol. Ther., 27, 2008, 9, s. 846-851.
Labels
Audiology Paediatric ENT ENT (Otorhinolaryngology)Article was published in
Otorhinolaryngology and Phoniatrics
2016 Issue 3
Most read in this issue
- Nanoparticles, Nanotoxicology, Nanomedicine: Definition of Terms, Perspectives in Otorhinolaryngology
- Secretory Carcinoma of Salivary Glands
- Orbitocellulitis - Potential Complication of Acute Rhinosinusitis Even with Proper Conservative Therapy
- Ganglioneuroma, a Rare Cause of Soft Neck Tissues Tumor in Adult Age