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HLA-DPB1 Gene Analysis in Haematopoietic Stem Cell Transplantations


Authors: M. Vraná;  M. Dobrovolná;  P. Cetkovský;  S. Nazarová;  H. Vondráčková;  P. Sedláček 1
Authors‘ workplace: Ústav hematologie a krevní transfuze, Praha ;  Klinika dětské hematologie a onkologie 2. LF UK a FNM, Praha 1
Published in: Čas. Lék. čes. 2006; 145: 126-129
Category: Original Article

Overview

Background.
The HLA-DPB1 gene is probably one of HLA class II genes affecting the haematopoietic stem cell transplantation. The aim of the study was to analyse the HLA-DPB1 gene and its match/mismatch in patients transplanted from unrelated HSC donors. The PCR-SSP method was used for the typing of the HLA system. 

Methods and Results.
The cohort consisted of 201 pairs of patient/unrelated HSC donor. Match in HLA-A, -B, -Cw, -DRB1 and -DQB1 (e.g. 10/10 match) was found in 81 pairs. The HLA-DPB1 was tested in them. 18 different HLA-DPB1 alleles were identified in this cohort. Complete match (e.g.12/12) was detected in 3% of the 201 analysed pairs only. 

Conclusions.
The probability of finding 12/12 matching unrelated HSC donor is limited due to the high percentage of mismatches and inaccessibility of previous HLA-DPB1 results. 

Key words:
haematopoietic stem cell transplantation, major histocompatibility system, HLA class I and HLA class II genotyping, locus, polymorphism, haplotype.


Labels
Addictology Allergology and clinical immunology Angiology Audiology Clinical biochemistry Dermatology & STDs Paediatric gastroenterology Paediatric surgery Paediatric cardiology Paediatric neurology Paediatric ENT Paediatric psychiatry Paediatric rheumatology Diabetology Pharmacy Vascular surgery Pain management Dental Hygienist

Article was published in

Journal of Czech Physicians


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