Immunohistochemistry of Biopsies of Allotransplanted Kidneys

Overview

Background.
Cellular rejection infiltration of the interstitium is the basic histological finding in biopsies of transplanted kidneys, and leukostasis in the muscular arteries and glomeruli is an important sign of exacerbating rejection. For better understanding and more accurate interpretation the authors used immunohistochemistry. Methods and Results. The authors examined 282 tissue specimens from 208 grafts using the two- or three-step immunoenzyme method with 28 mono- or polyclonal antibodies specific for a series of differentiation and activation leukocytic antigens, adhesion molecules and selected cytokines. In the compact component of the rejection infiltrate CD4 + lymphocytes with expression of CD 45 RA antigen predominated while in the disperse component there were mostly macrophages (CD68, 14, 11b); their number correlated significantly with the parenchymatous damage, similarly as intraarterial and glomerular accumulation. The disperse infiltrate and adherent cells expressed CD45 RO (rarely CD25) and integrin molecules of the series CD11 and CD49. CD57 + lymphocytes penetrated into the tubules but did not accumulate in the blood vessels. As to adhesive molecules of the „Ig superfamily“, CD106 (VCAM-1) was more important than CD54 (ICAM-1) and its arterial and mesangial expression correlated with the rejection damage. Evidence of cytokines (IL1, IL2, TNF alpha, beta) did provide neither unequivocal results nor correlations. Conclusions. Immunohistochemistry improves considerably the accuracy of bioptic evaluation of rejection nephropathy and some antigens (e.g., CD68, CD14, CD45 RO, CD57, CD106) are suitable for diagnostic practice. With their aid it is easier to evaluate the activity of rejection, assess the probability of vascular lesions in specimens without affected vessels and detect more sensitively intravascular stasis and adhesion of leukocytes.

Key words:
rejection, biopsy and immunohistochemistry of grafts, cell surface markers, adhesion molecules, T lymphocytes, macrophages.