#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Keratin fragments as a serological indicator in patients with a liver disease


Authors: J. Lata 1;  H. Mrlianová 2;  O. Stibůrek 2;  J. Nožička 3;  Z. Čermáková 4;  J. Gottwaldová 4;  P. Drozd 5
Authors‘ workplace: Interní klinika Lékařské fakulty Ostravské Univerzity a FN Ostrava, přednosta doc. MUDr. Arnošt Martínek, CSc. 1;  Interní gastroenterologická klinika Lékařské fakulty MU a FN Brno, pracoviště Bohunice, přednosta prof. MUDr. Aleš Hep, CSc. 2;  Histologická laboratoř Ústí nad Orlicí, přednosta primář MUDr. Jan Nožička, Ph. D. 3;  Oddělení klinické biochemie FN Brno, pracoviště Bohunice, přednosta doc. MUDr. Milan Dastych, CSc., MBA 4;  Katedra biologie a ekologie Přírodovědecké fakulty Ostravské univerzity v Ostravě, vedoucí katedry Mgr. Pavel Drozd, Ph. D. 5
Published in: Vnitř Lék 2011; 57(12): 1025-1028
Category: 70th birthday of prof. MUDr. Petr Dítě, DrSc.

Overview

Introduction:
Determination of M30 antigen appears to be a sensitive method for evaluation of hepatocyte apoptosis. The aim of the present project was to implement this technique, compare M30 values in patients with a liver disease and healthy controls and to identify potential relationships between M30 values and other parameters.

Methods:
M30 antigen was assessed in 25 patients with a liver disease and 30 healthy controls. These results were compared between patients with a liver disease and the controls and correlated to a range of clinical and laboratory values, including tissue polypeptide-specific antigen (TPS), in patients with a liver disease.

Results:
We found significant differences in M30 values between controls and patients with a liver disease. The only significant correlations were the correlation between M30 and ALT, highly significant correlation between M30 and AST and highly significant correlation between M30 and TPS.

Conclusion:
The M30 antigen is a sensitive indicator of liver damage. Considering the highly significant correlation with TPS, it can be assumed that in some liver diseases, TPS could serve as a clinically useful indicator of apoptosis.

Key words:
liver disease – M30 antigen – tissue polypeptide-specific antigen (TPS) – keratin fragments – hepatocyte apoptosis


Sources

1. R Development Core Team (2011). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna: Austria 2011. www.R-project.org.

2. Hůlek P, Dresslerová I. Steatóza a steatohepatitida u diabetika. Vnitř Lék 2011; 57: 364–367.

3. Brůha R. Nealkoholová steatóza a steatohepatitida. Vnitř Lék 2010; 56: 173–175.

4. Naveau S, Gaudé G, Asnacios A et al. Diagnostic and prognostic values of non-invasive biomarkers of fibrosis in patients with alcoholic liver disease. Hepatology 2009; 49: 97–105.

5. Tajmer P. Fibróza jater. In: Špičák J (ed.). Novinky v gastroenterologii a hepatologii. 1. vyd. Praha: Grada 2008: 35–46.

6. Moll R, Franke WW, Schiller DL et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982; 31: 11–24.

7. Fuchs E, Tyner AL, Guidice G et al. The human keratin genes and their differential expression. In: Sawyer RH (ed.). The Molecular and Developmental Biology of Keratins. New York: Academic Press 1987: 5–34.

8. Sundström BE, D’Amico Y, Brundell J. Development of a new Profiligen TPA IRMA assay using monoclonal anti-cytokeratin antibodies. Int J Biol Markers 1995; 10: 166–173.

9. Ueno T, Toi M, Linder S. Detection of epithelial cell death in the body by cytokeratin 18 measurement. Biomed Pharmacother 2005; 59 (Suppl 2): S359–S362.

10. Feldstein AE, Wieckowska A, Lopez AR et al. Cytokeratin-18 fragment levels as noninvasive biomarkers for nonalcoholic steatohepatitis: a multicenter validation study. Hepatology 2009; 50: 1072–1078.

11. Yilmaz Y, Dolar E, Ulukaya E et al. Soluble forms of extracellular cytokeratin 18 may differentiate simple steatosis from nonalcoholic steatohepatitis. World J Gastroenterol 2007; 13: 837–844.

12. Younossi ZM, Jarrar M, Nugent C et al. A Novel Diagnostic Biomarker Panel for Obesity-related Nonalcoholic Steatohepatitis (NASH). Obes Surg 2008; 11: 1430–1437.

13. Zetterberg A, Skern T, Schoberl E et al. Molecular characterization of TPS (Abstract). 22nd Meeting. Groningen: ISOBM 1994: 354.

14. Bremer K, Eklund G, Björklund B. Notable relationship between the level of tumor marker TPS in serum and survival in breast cancer. Anticancer Res 1996; 16: 905–909.

15. Glimelius B, Hoffman K, Einarsson R et al. Monitoring palliative chemotherapy in advanced gastrointestinal cancer using tissue polypeptide specific antigen (TPS) measurements. Acta Oncol 1996; 35: 141–148.

16. Klečka J, Pikner R, Toufarová P et al. Cytpkeratiny TPA, TPS, CYFRA 21-1 a UBC v séru a moči jako možné nádorové markery karcinomu močového měchýře. Ces Urol 2001; 1: 32–36.

17. http://www.cskb.cz/res/file/doporuceni/TM/TM_tab3.pdf.

18. Gonzalez-Quintela A, Abdulkader I, Campos J et al. Serum Levels of Keratin-18 Fragments (Tissue Polypeptide-Specific Antigen TPS) Are Correlated with Hepatocyte Apoptosis in Alcoholic Hepatitis. Dig Dis Sci 2009; 54: 648–653.

19. Gonzalez-Quintela A, Abdulkader I, Otero E et al. Relationship between serum concentrations of keratin-18 and apoptosis in chronic hepatitis C. Clin Chem Lab Med 2010; 48: 897–899.

Labels
Diabetology Endocrinology Internal medicine

Article was published in

Internal Medicine

Issue 12

2011 Issue 12

Most read in this issue
Topics Journals
Login
Forgotten password

Enter the email address that you registered with. We will send you instructions on how to set a new password.

Login

Don‘t have an account?  Create new account

#ADS_BOTTOM_SCRIPTS#