Isolation and immunology identification of spermagglutinating antibodies from human serum

Czech version

Authors: J. Cibulka ¹; Z. Ulčová-Gallová ¹; M. Balvín ²; K. Bibková ¹; Z. Mičanová ¹
Authors‘ workplace: Gynekologicko-porodnická klinika LF UK a FN Plzeň, přednosta doc. MUDr. Z. Rokyta, CSc. ¹; Ústav soudního lékařství LF UK, Plzeň, přednostka doc. MUDr. H. Kvapilová, CSc. ²
Published in: Ceska Gynekol 2009; 74(3): 201-208

Overview

Objective:
Isolation of spermagglutinating antibodies and their assesment.

Design:
Retrospective study.

Setting:
Special consulting for reptoduction immunology, Department of Obstetrics and Gynecology, Charles University and Faculty Hospital, Plzeň.

Methods:
Fractionation of serum samples by liquid exclusion chromatography, examination of full sera and their chromatographic fractions by Friberg teste (Tray Agglutination Test – TAT), indirect antiimmunoglobulin reaction test (i-MAR test) and by supplementar radial immunodiffusiona (RID).

Results:
Isolation of spermagglutinating fractions of antisperm antibodies positive sera preserved spermagglutinating aktivity and confirmed great spermagglutinating potential of IgM.

Conclusion:
According to assesment of the presence of IgG and IgG we reported possible states of immunisation: actual immunisation with IgM activity, perpetual stimulation (IgG and IgM) and, finaly, anamnestic titres in IgG. These findings can help us to choose an optimal way of treatment. Excluding gel chromatography is suitable method for serum proteins fractionation, but not their identification – presence of antisperm antibodies does not affect the chromatographic spectrum, nor the RID patterns.

Key words:
reproduction immunology, infertility, antisperm antibodies, i-MAR test, TAT test, excluding gel chromatography.

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