Use of Frozen Sperm Cells in the Microaggulation Test

Czech version

Authors: P. Seifertová; Z. Ulčová-Gallová; P. Panzner ^*; K. Křižanovská; Z. Rokyta
Authors‘ workplace: Gynekologicko-porodnická klinika LF UK a FN v Plzni, přednosta doc. MUDr. Z. Rokyta, CSc.
Published in: Ceska Gynekol 2000; (3): 138-141
Category:

Overview

Objective:
The aim of this study was to find out the behavior of sperm cryopreserved in microag-glutination test (TAT).Methods: TAT using native sperm pertains to the screening methods in spermagglutinating anti-bodies. Spermatozoa cryopreserved in egg-yolk medium, just in seminal plasma or in the commer-cial made medium - Medi - Cult (Denmark) were used in this study.Setting: Department of Gynaecology and Obstetrics, Institut of Clinical Immunology and Allergo-logy Medical Faculty of Charles University, Pilsen.Results: 367 tests were performed together. It includes 108 examinations with native spermatozoa,59 with spermcryopreserved in egg-yolk medium, 109 with spermcryopreserved just in seminalplasma and 91 in Medi-Cult medium. The correspondance of results with native and cryopreser-ved sperm was found in 24% for egg-yolk medium, in 69% for seminal plasma and in 77% forcommercial medium Medi-Cult.Conclusion: The results show that spermcryopreservation in Medi-Cult medium or just in theseminal plasma are able to be used in TAT for detection of spermagglutinating antibodies.

Key words:
microagglutinating test, sperm, cryopreservation, infertility

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