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Determination of varenicline in the preparation Champix® with the use of two-dimensional capillary electrophoresis in connection with UV detection


Authors: Juraj Piešťanský;  Katarína Maráková;  Lucia Veizerová;  Jaroslav Galba;  Peter Mikuš
Authors‘ workplace: Bratislava, Slovenská republika ;  Katedra farmaceutickej analýzy a nukleárnej farmácie FaF UK
Published in: Čes. slov. Farm., 2013; 62, 270-275
Category: Original Articles

Overview

This paper deals with an analytical method based on two dimensional capillary electrophoresis (CITP-CZE coupling) with simple UV-detection for the determination of a highly effective drug – varenicline. The method was elaborated with the possibility of its future connection with an advanced detection ending – mass spectrometry. The electrolytes for the CITP (LE = 10 mM NH4Ac + 5 mM HAc + 0,05% m-HEC; TE = 10 mM HAc) and CZE (BGE = 20 mM HAc) separation of varenicline were selected considering such requirements. The UV detector was set at the constant wavelength of 237 nm. The presented CITP-CZE-UV combination enabled rapid and effective evaluation of varenicline in the dosage forms with LOD 5.92 ng/ml.

Keywords:
nicotine, varenicline, two dimensional capillary electrophoresis, dosage forms, pharmacy


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