The effect of three different elicitors on sanguinarine production in suspension cultures of a low-morphine variety of the opium poppy (Papaver somniferum L.)
Authors:
Andrea Balážová; Víťazoslava Blanáriková; František Bilka; Andrea Bilková; Hana Kiňová Sepová
Authors‘ workplace:
Univerzita Komenského v Bratislave, Farmaceutická fakulta, Katedra bunkovej a olekulárnej biológie liečiv, Slovenská republika
Published in:
Čes. slov. Farm., 2011; 60, 237-240
Category:
Original Articles
Overview
This paper is focused on the evaluation of the effect of three different elicitors on sanguinarine production in suspension cultures derived from a low-morphine variety of the opium poppy. The elicitors CdCl2, methyl jasmonate and a homogenate from the phytopathogenic fungus Botrytis cinerea have been administered into the growth media of 11-day-old suspension cultures. Their effect on the production of sanguinarine was monitored after 24, 48 and 72 h of exposure. The highest sanguinarine production was observed after 48 h of exposure to the biotic elicitor Botrytis cinerea, which increased the sanguinarine production 5.3-times. In the presence of CdCl2 at a concentration of 1 mmol.l-1 the content of sanguinarine increased linearly in suspension cultures and after 72 h of elicitation it was 2.3-times higher than in the non-elicited cultures. Opium poppy suspension cultures responded to methyl jasmonate elicitation by a moderate increase in sanguinane production after 24 and 48 h. A significant increase in the production of this secondary metabolite in cell suspension cultures was observed after 72 h of exposure and it was 3-times higher than the production of sanguinarine in non-elicited cultures.
Key words:
sanguinarine – methyl jasmonate – Botrytis cinerea – CdCl2 – elicitation – opium poppy
Sources
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Labels
Pharmacy Clinical pharmacologyArticle was published in
Czech and Slovak Pharmacy
2011 Issue 5
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