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Inhibiční aktivita vybraných ß-karbolínových alkaloidů na enzymy acetylcholinesterasu a butyrylcholinesterasu


Authors: Zuzana Kršková;  Jan Martin;  Jaroslav Dušek
Authors‘ workplace: Department of Pharmacognosy, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Czech Republic
Published in: Čes. slov. Farm., 2011; 60, 125-131
Category: Original Articles

Overview

Tato práce se zabývá testováním inhibiční aktivity ß-karbolínových alkaloidů na aktivitu enzymů acetylcholinesterasy (ACHE) a butyrylcholinesterasy (BUCHE) s použitím „Fast Blue B salt“ testu na TLC desce a Ellmanova testu provedeném na spektrofotometru. Také bylo zkoumáno, jak dimethylsulfoxid (DMSO), který byl použit ve směsi s vodou jako rozpouštědlo, ovlivňuje aktivitu enzymů a alkaloidů. Z výsledků vyplývá, že nejvyšší inhibiční aktivitu na ACHE má s použitím eserinu jako referenční látky harmin ve formě báze i soli, a to jak v prostředí vodném, tak i ve směsi DMSO s vodou. Nejvyšší inhibiční aktivitu na BUCHE vykazuje ve vodném prostředí harmalol ve formě soli, ve směsi DMSO s vodou je nejaktivnější harmin ve formě báze i soli. Ukázalo se, že DMSO značně ovlivňuje nejen aktivitu enzymů, ale také alkaloidů.

Klíčová slova:
ß-karbolínové alkaloidy – ACHE – BUCHE – DMSO – Ellmanův test – test „Fast Blue B salt“


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