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Studies of local anesthetics Part: 187 Extraction of 1-methyl-2-piperidinoethylesters of alkoxyphenylcarbamic acids from human plasma by imprinted polymers and its comparison with classic solid phase extraction


Authors: J. Čižmárik 2;  M. Lachová 1;  J. Lehotay 1;  I. Skačáni 1
Authors‘ workplace: STU v Bratislave, Fakulta chemickej a potravinárskej technológie, Ústav analytickej chémie 1;  Univerzita Komenského Bratislava, Farmaceutická fakulta, Katedra farmaceutickej chémie 2
Published in: Čes. slov. Farm., 2009; 58, 78-82
Category: Review Articles

Overview

Molecularly imprinted polymers for 1-methyl-2-piperidinoethylester of 2-methoxyphenylcarbamic acid (template) have been synthesised. Acrylamide and methacrylic acids, respectively, were used as the functional monomers. Imprinted polymers were used as the sorbents for solid-phase extraction (MISPE). The capacity and selectivity of prepared imprinted polymers were investigated with the use of some derivatives of 1-methyl-2-piperidinethylesters of alkoxyphenylcarbamic acids. The non-imprinted (blank) polymers were prepared by the same way without a template to study the non-specific interactions. The molecularly imprinted polymer prepared from methacrylic acid was used to preconcentrate 1-methyl-2-piperidinoethylester of 2-methoxyphenylcarbamic acid and its analogues from spiked human plasma (1 μg in 1 ml of plasma).

Key words:
esters of alkoxyphenylcarbamic acids – HPLC – imprinted polymers – solid phase extraction


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