SNHG7 a FAIM2 jsou ve tkáni kolorektálního karcinomu up-regulovány a koexprimovány

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Authors: Farinaz Ziaee ¹; Mohammadreza Hajjari ¹; Reza Seyed Kazeminezhad ¹; Mehrdad Behmanesh ²
Authors‘ workplace: Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran ¹; Department of Genetics, Faculty of Biological Science, Tarbiat Modares University (TMU), Tehran, Iran ²
Published in: Klin Onkol 2020; 33(6): 445-449
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Overview

Východiska: Očekává se, že během několika let celosvětově vzroste výskyt kolorektálního karcinomu (CRC) o 60 %. Přes pokroky v chirurgických technikách a v chemoterapii značná část pacientů s CRC vykazuje špatnou odpověď na léčbu. To jsou fakta, která svědčí o důležitosti identifikace molekulárních biomarkerů jakožto potenciálních léčebných cílů. Iniciace, růstu, progrese a metastazování nádorů, jako je např. CRC, se účastní dlouhé nekódující RNA (lnc RNA). Tato skupina nekódujících RNA je tedy známa jako biomarker pro diagnózu a prognózu tohoto nádorového onemocnění. Materiál a metody: V této experimentální studii byla provedena extrakce celkové RNA z tkání, syntéza komplementární DNA a kvantitativní polymerázová řetězová reakce v reálném čase (qRT-PCR). Pro kvantifikaci míry exprese lncRNA-SNHG7 a FAIM2 byla použita komparativní metoda „cycle threshold“. Relativní množství lncRNA-SNHG7 a FAIM2 bylo vypočítáno pomocí vztahu 2^-^DD^CT. Výsledky: Z výsledků qRT-PCR vyplynulo, že v porovnání s normálními tkáněmi bylo ve tkání CRC zvýšené množství SNHG7, který byl jako lncRNA popsán teprve nedávno, a FAIM2. Závěr: Naše studie svědčí o potenciální důležitosti exprese SNHG7 and FAIM2 a o důležitosti provedení více studií v budoucnu.

Klíčová slova:

lncRNA-SNHG7 – FAIM2 – kolorektální karcinom

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