New trends in immunochemistry

Czech version

Authors: P. Štern
Authors‘ workplace: Mimoňská 637/16, 190 00 Praha ⁹
Published in: Klin. Biochem. Metab., 24, 2016, No. 4, p. 164-169

Overview

The educational article deals with some procedures that extended applications of immunochemistry in the last decade. Fluorescence immunochromatography combines advantages of chromatographic methods and immunoassays with fluorophor labels. Flow horizontal detection using a fluorophor such as Alexa 647 or sulfo-rhodamine B (also incorporated in liposomes) is most widely used. Nanoparticles combined with europium ions, silica coated or semiconductor nanocrystals are also used as fluorophors. Immunochromatography in connection with tandem mass spectrometry has been used in research rather than in clinical laboratory practice. For enrichment of determined analyte immunoaffinity chromatography is used. For standardization incorporating stable-isotopes into the specific peptides of interest is applied. Implementing of ultrasensitive methods, such as application of giant electrical magnetoresistive sensor or field-effect transistor technology, is in a pilot stage.

Keywords:
fluorescence immunochromatography, mass spectrometry, giant electrical magnetoresistive sensor, field-effect transistor.

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Article was published in

Clinical Biochemistry and Metabolism

2016 Issue 4